Airborne bacterial communities in residences: similarities and differences with fungi.
Airborne bacterial communities in residences: similarities and differences with fungi.
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DOI：
10.1371/journal.pone.0091283
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bruns TD
中科院分区：
文献类型：
--
作者：
Adams RI;Miletto M;Lindow SE;Taylor JW;Bruns TD
对室内空气的遗传分析发现了丰富的微生物存在，但很少在同一样本中同时检测细菌和真菌成分。在这里，我们提出了一项研究，该研究检查了室内和室外空气中被动停留的微生物在一段离散的时间段内的细菌成分，其中真菌成分已经被报道。一所大学-住房综合体内不同住宅的家庭−客厅、卧室、浴室、厨房和阳台−周围的五个常见位置被允许在两个时间点被动降尘一个月，一次在夏天，另一次在冬天。我们从这些样本中扩增了细菌16S rRNA基因，并对其进行了高通量测序。与真菌的OTU丰富度一样，细菌的OTU丰富度在室外高于室内，并且在不同的室内类型中是不变的。真菌组成主要由季节和居室构成，细菌组成因居室和居室类型而异。来自室外来源的细菌，如神经鞘氨醇单胞菌和葡萄球菌，在阳台样本中占很大比例，而与人类有关的分类群占室内样本的比例很大。在室内也观察到了丰富的室外细菌分类群，但情况并非如此；这不同于真菌，在室内丰富的分类群在室外也有很好的表现。此外，细菌组成与地理距离存在部分关联，即使相隔几百米的样本往往比距离较近的太空样本具有更大的组成差异，真菌也存在这种模式。这些数据表明，虽然室内细菌和真菌的室外来源在空间和时间上都不同，但人类对室内细菌生物气溶胶提供了强烈和均质的影响，这是在真菌中没有观察到的模式。
Genetic analysis of indoor air has uncovered a rich microbial presence, but rarely have both the bacterial and fungal components been examined in the same samples. Here we present a study that examined the bacterial component of passively settled microbes from both indoor and outdoor air over a discrete time period and for which the fungal component has already been reported. Dust was allowed to passively settle in five common locations around a home − living room, bedroom, bathroom, kitchen, and balcony − at different dwellings within a university-housing complex for a one-month period at two time points, once in summer and again in winter. We amplified the bacterial 16S rRNA gene in these samples and analyzed them with high-throughput sequencing. Like fungal OTU-richness, bacterial OTU-richness was higher outdoors then indoors and was invariant across different indoor room types. While fungal composition was structured largely by season and residential unit, bacterial composition varied by residential unit and room type. Bacteria from putative outdoor sources, such as Sphingomonas and Deinococcus, comprised a large percentage of the balcony samples, while human-associated taxa comprised a large percentage of the indoor samples. Abundant outdoor bacterial taxa were also observed indoors, but the reverse was not true; this is unlike fungi, in which the taxa abundant indoors were also well-represented outdoors. Moreover, there was a partial association of bacterial composition and geographic distance, such that samples separated by even a few hundred meters tended have greater compositional differences than samples closer together in space, a pattern also observed for fungi. These data show that while the outdoor source for indoor bacteria and fungi varies in both space and time, humans provide a strong and homogenizing effect on indoor bacterial bioaerosols, a pattern not observed in fungi.
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影响因子：
5.1
作者：
Flores, Gilberto E.;Bates, Scott T.;Fierer, Noah
通讯作者：
Fierer, Noah
影响因子：
3.7
作者：
Flores GE;Bates ST;Knights D;Lauber CL;Stombaugh J;Knight R;Fierer N
通讯作者：
Fierer N
影响因子：
3.7
作者：
Adams RI;Miletto M;Taylor JW;Bruns TD
通讯作者：
Bruns TD
影响因子：
3.7
作者：
Dunn RR;Fierer N;Henley JB;Leff JW;Menninger HL
通讯作者：
Menninger HL
影响因子：
4.4
作者：
Bowers, Robert M.;Sullivan, Amy P.;Fierer, Noah
通讯作者：
Fierer, Noah