Genomic processes underlying rapid adaptation of a natural Chironomus riparius population to unintendedly applied experimental selection pressures
Genomic processes underlying rapid adaptation of a natural Chironomus riparius population to unintendedly applied experimental selection pressures
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自然摇蚊群体快速适应无意施加的实验选择压力的基因组过程
DOI：
10.1111/mec.15347
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发表时间：
2019
影响因子：
4.9
通讯作者：
Foucault Q
中科院分区：
文献类型：
--
作者：
Pfenninger M ;Foucault Q
进化和重新排序（E&R）研究是研究快速适应过程中基因组过程的有用工具，例如，在适应全球气候变化的框架内。我们应用不同的热制度，以naturalChironomus riparius（双翅目）人口的E&R框架，以推断其快速热适应的进化潜力。我们将两个重复暴露于三种温度（14°C，20°C和26°C）超过两年，因此实验分别持续22，44或65代。这两个较高的温度提出了一个先验的温和，分别强选择压力。生命周期适应性测试显示，对温度状况没有明显的适应，但所有六个重复的共同适应可能是由于饲养条件，可能是幼虫密度和水质增加。基因组分析显示，所有重复中均存在强的全基因组选择性应答（选定SNP的平均值= 0.305）。在所有重复样本中，该基因组应答在所有基因组水平上均显著相似（SNP、10 kb窗口、基因、外显子、等位基因频率变化升高区域[REA]）。暴露于相同温度的重复之间的交叉点是不显著的或代表性不足。这证实了对相同的选择压力的选择性响应，然而，不是对热状态的选择性响应。与每个REA具有最高选择系数的SNP最接近的基因富集了与离子转运、转录调节和信号转导相关的GO术语，这支持了假定的作用选择压力。我们的研究表明，通过全基因组和可能的多基因选择对河岸锦鸡儿的遗传变异进行快速适应的进化潜力。然而，由于不可能准确地预测在进化实验中的代理选择制度，我们讨论了发人深省的观点推断自然种群的进化潜力，这种方法。
Evolve and Resquence (E&R) studies are a useful tool to study genomic processes during rapid adaptation, e.g., in the framework of adaptive responses to global climate change. We applied different thermal regimes to a naturalChironomus riparius(Diptera) population in an E&R framework to infer its evolutionary potential for rapid thermal adaptation. We exposed two replicates to three temperatures each (14°C, 20°C and 26°C) for more than two years, the experiment thus lasting 22, 44 or 65 generations, respectively. The two higher temperatures presented a priori moderate, respectively strong selection pressures. Life‐cycle fitness tests revealed no appreciable adaptation to thermal regimes but a common adaptation of all six replicates probably due to the rearing conditions, presumably increased larval density and water quality. Genomic analyses showed a strong, genome‐wide selective response in all replicates (mean s of selected SNPs = 0.305). This genomic response was significantly similar at all genomic levels among all replicates (SNPs, 10 kb windows, genes, exons, regions of elevated allele frequency change [REA]). The intersections among the replicates exposed to the same temperature were either insignificant or underrepresented. This confirmed a selective response to identical selection pressure(s), however, not to thermal regime. Genes closest to the SNP with the highest selection coefficient per REA were enriched for GO terms related to ion transport, regulation of transcription and signal transduction, which supported the presumed acting selection pressures. Our study showed the evolutionary potential for rapid adaptation by genome‐wide and probably polygenic selection on standing genetic variation inC. riparius. However, because of the impossibility to accurately predict the acting selective regime in evolutionary experiments, we discuss the sobering perspectives for inferring the evolutionary potential of natural populations with this approach.
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影响因子：
4.1
作者：
Griffith MB
通讯作者：
Griffith MB
咸水甲虫对急性热和渗透胁迫的致死和亚致死行为反应
影响因子：
2.2
作者：
S. Pallarés;P. Arribas;V. Céspedes;A. Millán;J. Velasco
通讯作者：
J. Velasco
猪笼草的生态学和进化： 1. 种群动态以及实验室对食物和人口密度的反应
DOI：
--
发表时间：
1975
期刊：
Evolution; international journal of organic evolution
影响因子：
--
作者：
C. Istock;S. Wasserman;Harold Zimmer
通讯作者：
Harold Zimmer
影响因子：
2.6
作者：
Foucault, Quentin;Wieser, Andreas;Pfenninger, Markus
通讯作者：
Pfenninger, Markus
影响因子：
3.8
作者：
Porcelli D;Butlin RK;Gaston KJ;Joly D;Snook RR
通讯作者：
Snook RR