A qualitative proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.).
A qualitative proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.).
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DOI：
10.1038/s41598-018-26676-y
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发表时间：
2018-05-29
影响因子：
4.6
通讯作者：
Zheng X
中科院分区：
文献类型：
--
作者：
Liu K;Yuan C;Li H;Chen K;Lu L;Shen C;Zheng X
组蛋白的赖氨酸巴豆酰化是最近发现的具有多种细胞功能的翻译后修饰。然而，没有关于果实细胞中非组蛋白蛋白质赖氨酸巴豆酰化的信息。使用高分辨率 LC-MS/MS 结合高灵敏度的免疫亲和抗体分析，对木瓜果实 (Carica papaya L.) 进行了整体巴豆酰化蛋白质组分析。总共发现了 2,120 个具有 5,995 个赖氨酸巴豆酰化位点的蛋白质，其中鉴定出了 8 个保守基序。生物信息学分析将巴豆酰化蛋白与多种代谢途径联系起来，包括抗生素的生物合成、碳代谢、氨基酸的生物合成和糖酵解。特别是，鉴定出参与氨基酸代谢各种途径的 40 种巴豆酰化酶，表明巴豆酰化在调节氨基酸代谢中具有潜在的保守功能。在涉及激素信号传导和细胞壁相关途径的蛋白质中发现了许多巴豆酰化位点。我们的综合巴豆酰化蛋白质组表明木瓜中赖氨酸巴豆酰化具有多种功能。
Lysine crotonylation of histone proteins is a recently-identified post-translational modification with multiple cellular functions. However, no information about lysine crotonylation of non-histone proteins in fruit cells is available. Using high-resolution LC-MS/MS coupled with highly sensitive immune-affinity antibody analysis, a global crotonylation proteome analysis of papaya fruit (Carica papaya L.) was performed. In total, 2,120 proteins with 5,995 lysine crotonylation sites were discovered, among which eight conserved motifs were identified. Bioinformatic analysis linked crotonylated proteins to multiple metabolic pathways, including biosynthesis of antibiotics, carbon metabolism, biosynthesis of amino acids, and glycolysis. particularly, 40 crotonylated enzymes involved in various pathways of amino acid metabolism were identified, suggesting a potential conserved function for crotonylation in the regulation of amino acid metabolism. Numerous crotonylation sites were identified in proteins involved in the hormone signaling and cell wall-related pathways. Our comprehensive crotonylation proteome indicated diverse functions for lysine crotonylation in papaya.
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影响因子：
56.9
作者：
Choudhary, Chunaram;Kumar, Chanchal;Mann, Matthias
通讯作者：
Mann, Matthias
通过 SlymiR157 调节 LeSPL-CNR 表达影响番茄果实成熟
影响因子：
4.6
作者：
Chen W;Kong J;Lai T;Manning K;Wu C;Wang Y;Qin C;Li B;Yu Z;Zhang X;He M;Zhang P;Gu M;Yang X;Mahammed A;Li C;Osman T;Shi N;Wang H;Jackson S;Liu Y;Gallusci P;Hong Y
通讯作者：
Hong Y
DOI：
10.1111/tpj.12766
发表时间：
2015-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Snowden CJ;Thomas B;Baxter CJ;Smith JA;Sweetlove LJ
通讯作者：
Sweetlove LJ
影响因子：
4.4
作者：
Liu K;Yuan C;Feng S;Zhong S;Li H;Zhong J;Shen C;Liu J
通讯作者：
Liu J
影响因子：
6.9
作者：
Bastías A;Yañez M;Osorio S;Arbona V;Gómez-Cadenas A;Fernie AR;Casaretto JA
通讯作者：
Casaretto JA