A modified adjusting delay task to assess impulsive choice between isocaloric reinforcers in non-deprived male rats: effects of 5-HT₂A/C and 5-HT₁A receptor agonists.
A modified adjusting delay task to assess impulsive choice between isocaloric reinforcers in non-deprived male rats: effects of 5-HT₂A/C and 5-HT₁A receptor agonists.
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DOI：
10.1007/s00213-011-2517-8
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发表时间：
2012-01
影响因子：
3.4
通讯作者：
Cottone, Pietro
中科院分区：
文献类型：
--
作者：
Blasio, Angelo;Narayan, Aditi R.;Kaminski, Barbara J.;Steardo, Luca;Sabino, Valentina;Cottone, Pietro
现有的冲动动物模型经常使用食物限制来增加受试者的动机。此外，评估冲动选择的行为任务通常涉及使用不同卡路里含量的增强剂。这些因素代表了能量动态平衡的限制，这可能会混淆对结果的解释，并限制这些模型的适用性。本研究的目的是验证一种改进的调整延迟任务的面子和收敛效度，该任务评估了自由喂养大鼠在等热量钢筋之间的冲动选择。用雄性Wistar大鼠(n=18)评价“超糖精”溶液(1.5%葡萄糖/0.4%糖精)对1.5%葡萄糖溶液的偏好和增强效果。另一组大鼠(n=24)接受了改进的调整延迟任务的训练，该任务包括在立即给药的葡萄糖溶液和可变延迟后给药的过糖精溶液之间重复选择。为了从药理学上验证这项任务，研究了5-HT2A/C受体激动剂(±)-1-(2，5-dimethoxy-4-iodophenyl)-2-aminopropane[(±)-DOI]和5-HT1A受体激动剂(±)-8-羟基-2-(二丙氨基)四氢吡喃[(±)-8-OH-DPAT]对冲动选择的影响。糖精是高度强化的，并且在所有受试者中均优于葡萄糖溶液。老鼠很快学会了这个任务，冲动是一种非常稳定和始终如一的特征。DOI和8-OH-DPAT显著增加了调整延迟任务中的冲动选择，且呈剂量依赖性。我们验证了一项啮齿动物冲动选择的任务，它消除了典型的能量平衡限制，因此，在临床前喂养和肥胖研究中开辟了研究冲动的新途径。
Existing animal models of impulsivity frequently use food restriction to increase subjects’ motivation. In addition, behavioral tasks that assess impulsive choice typically involve the use of reinforcers with dissimilar caloric content. These factors represent energy-homeostasis limitations, which may confound the interpretation of results and limit the applicability of these models. This study was aimed at validating face and convergent validities of a modified adjusting delay task, which assesses impulsive choice between isocaloric rein-forcers in ad libitum fed rats. Male Wistar rats (n=18) were used to assess the preferredness and reinforcing efficacy of a “supersaccharin” solution (1.5% glucose/0.4% saccharin) over a 1.5% glucose solution. A separate group of rats (n=24) was trained in a modified adjusting delay task, which involved repeated choice between the glucose solution delivered immediately and the supersaccharin solution delivered after a variable delay. To pharmacologically validate the task, the effects of the 5-HT2A/C receptor agonist (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane [(±)-DOI] and the 5-HT1A receptor agonist (±)-8-hydroxy-2-(dipropylamino)tetralin hydrobromide [(±)-8-OH-DPAT] on impulsive choice were then evaluated. Supersaccharin was highly reinforcing and uniformly preferred over the glucose solution by all subjects. Rats quickly learned the task, and impulsivity was a very stable and consistent trait. DOI and 8-OH-DPAT significantly and dose dependently increased impulsive choice in this modified adjusting delay task. We validated a rodent task of impulsive choice, which eliminates typical energy-homeostasis limitations and, therefore, opens new avenues in the study of impulsivity in preclinical feeding and obesity research.
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