Identification and characterization of serovar-independent immunogens in Actinobacillus pleuropneumoniae.
Identification and characterization of serovar-independent immunogens in Actinobacillus pleuropneumoniae.
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DOI：
10.1186/s13567-017-0479-5
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发表时间：
2017-11-09
影响因子：
4.4
通讯作者：
Bojesen AM
中科院分区：
文献类型：
--
作者：
Antenucci F;Fougeroux C;Bossé JT;Magnowska Z;Roesch C;Langford P;Holst PJ;Bojesen AM
尽管采取了许多措施来预防疾病，但胸膜肺炎放线杆菌（A.胸膜肺炎）仍然是猪胸膜肺炎的主要原因，导致全世界养猪业的经济损失。在本文中，我们描述了利用反向疫苗学方法选择和体外测试血清型非依赖性A。胸膜肺炎免疫原。在3株A.使用以下参数确定属于不同血清型的胸膜肺炎菌株：预测的外膜亚细胞定位; ≤ 1个跨膜螺旋;蛋白质序列中存在信号肽;在所有已知的A.胸膜肺炎基因组;与其他充分表征的因子具有关于免疫原性/保护潜力的相关数据的同源性。使用这种方法，我们选择了蛋白质ApfA和VacJ表达和进一步表征，无论是在电脑和体外。此外，我们还分析了A.胸膜肺炎MIDG 2331作为潜在的免疫原，并比较了degS和nlpI的缺失，以增加与亲本菌株相比的OMV产量。我们的研究结果表明，ApfA和VacJ是高度保守的蛋白质，在所有A.测试的胸膜肺炎血清型。此外，OMV、ApfA和VacJ在体外显示出具有高免疫原性潜力。这些发现支持所使用的免疫原选择方案，并表明OMV与ApfA和VacJ一起沿着，可以代表预防A.胸膜肺炎以血清型非依赖性方式感染。尽管如此，该假设本质上是预测性的，并且需要在相关动物模型中进行体内测试以验证其有效性。本文的在线版本（10.1186/s13567-017-0479-5）包含补充材料，可供授权用户使用。
Despite numerous actions to prevent disease, Actinobacillus pleuropneumoniae (A. pleuropneumoniae) remains a major cause of porcine pleuropneumonia, resulting in economic losses to the swine industry worldwide. In this paper, we describe the utilization of a reverse vaccinology approach for the selection and in vitro testing of serovar-independent A. pleuropneumoniae immunogens. Potential immunogens were identified in the complete genomes of three A. pleuropneumoniae strains belonging to different serovars using the following parameters: predicted outer-membrane subcellular localization; ≤ 1 trans-membrane helices; presence of a signal peptide in the protein sequence; presence in all known A. pleuropneumoniae genomes; homology with other well characterized factors with relevant data regarding immunogenicity/protective potential. Using this approach, we selected the proteins ApfA and VacJ to be expressed and further characterized, both in silico and in vitro. Additionally, we analysed outer membrane vesicles (OMVs) of A. pleuropneumoniae MIDG2331 as potential immunogens, and compared deletions in degS and nlpI for increasing yields of OMVs compared to the parental strain. Our results indicated that ApfA and VacJ are highly conserved proteins, naturally expressed during infection by all A. pleuropneumoniae serovars tested. Furthermore, OMVs, ApfA and VacJ were shown to possess a high immunogenic potential in vitro. These findings favour the immunogen selection protocol used, and suggest that OMVs, along with ApfA and VacJ, could represent effective immunogens for the prevention of A. pleuropneumoniae infections in a serovar-independent manner. This hypothesis is nonetheless predictive in nature, and in vivo testing in a relevant animal model will be necessary to verify its validity. The online version of this article (10.1186/s13567-017-0479-5) contains supplementary material, which is available to authorized users.
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影响因子：
9.4
作者：
Bossé JT;Li Y;Sárközi R;Gottschalk M;Angen Ø;Nedbalcova K;Rycroft AN;Fodor L;Langford PR
通讯作者：
Langford PR
影响因子：
6.4
作者：
Chiers, K;van Overbeke, I;Haesebrouck, F
通讯作者：
Haesebrouck, F
影响因子：
2.1
作者：
Negrete-Abascal, E;García, RM;de la Garza, M
通讯作者：
de la Garza, M
影响因子：
3.2
作者：
McBroom, Amanda J.;Johnson, Alexandra P.;Kuehn, Meta J.
通讯作者：
Kuehn, Meta J.
DOI：
10.1073/pnas.0805532107
发表时间：
2010-02-16
影响因子：
11.1
作者：
Chen, David J.;Osterrieder, Nikolaus;Putnam, David
通讯作者：
Putnam, David