Magnetic hydrophilic polymer-based apta-sensing probe for sensitive detection of fetuin-A in serum
Magnetic hydrophilic polymer-based apta-sensing probe for sensitive detection of fetuin-A in serum
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基于磁性亲水聚合物的适体传感探针用于灵敏检测血清中的胎球蛋白-A
DOI：
10.1016/j.snb.2022.132152
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发表时间：
2022-06
影响因子：
8.4
通讯作者：
Feng Qu
中科院分区：
文献类型：
--
作者：
Liping Zhao;Muhammad Irfan;Xiaomin Zhang;Ge Yang;Yao Ma;Bo Wei;Feng Qu
提出了一种基于磁性亲水聚合物（MHP）的新型apta传感探针，用于检测血清基质中的胎球蛋白A。简而言之，MHP是由亲水性单体和磁性纳米颗粒通过自由基聚合方法合成的亲水性聚合物修饰的磁性纳米颗粒。首先通过毛细管电泳-SELEX筛选出针对胎球蛋白A的适体，然后与FAM标记的适体互补序列（CS）杂交形成双链体结构，最后将其偶联在MHP上形成MHP适体传感探针（MHPA）。该探针以具有亲水性的MHP为分离载体，以核酸适体结合胎球蛋白A，以FAM标记的CS为信号探针。当存在胎球蛋白-A时，由于胎球蛋白-A-适体和适体-CS之间的竞争性结合，CS逃逸并且可以被检测到。由于适体对蛋白质的特异性亲和力和聚合物的亲水性的协同作用，MHPA对胎球蛋白-A表现出灵敏和选择性的检测。在最佳条件下，线性范围为5.0 nM ~ 4.0 μM，检测限为4.4 nM。该方法具有良好的稳定性和选择性，已成功应用于血清中胎球蛋白A的检测。所制备的亲水性聚合物辅助apta传感探针具有简便、灵敏的特点，为蛋白质的高效检测提供了新的思路。·提出了一种基于磁性亲水聚合物（MHP）的新型apta传感探针，用于检测胎球蛋白-A。·由于apatmer和聚合物的协同作用，该探针对胎球蛋白-A表现出灵敏和选择性的检测。该探针与文献报道的方法相容性良好，可用于血清中胎球蛋白A的检测。
A new apta-sensing probe based on magnetic hydrophilic polymer (MHP) was proposed for simple and sensitive detection of fetuin-A in serum matrix. Briefly, MHP was the hydrophilic polymer modified magnetic nanoparticles, which was synthesized through free radical polymerization method with hydrophilic monomers and magnetic nanoparticles. Then, aptamer against fetuin-A first selected through capillary electrophoresis-SELEX was hybridized with FAM-labeled aptamer complementary sequence (CS) to form duplex structure, which was finally conjugated on MHP to form MHP apta-sensing probe (MHPA). In this probe, MHP with hydrophilic property was employed as separation carrier, aptamer was used for fetuin-A binding and FAM-labeled CS as signal probe. With the presence of fetuin-A, CS escaped and could be detected because of the competitive binding between fetuin-A-aptamer and aptamer-CS. Because of the synergistic effect of specific affinity of aptamer on protein and hydrophilic property of the polymer, MHPA exhibited sensitive and selective detection for fetuin-A. Under optimal conditions, the linear range was 5.0 nM-4.0 μM with the detection limit of 4.4 nM. What’s more, MHPA engaged with good stability and selectivity had been successfully applied in the detection of fetuin-A in serum samples. The as prepared hydrophilic polymer assistant apta-sensing probe with facile and sensitive properties, provides novel explication for efficient protein detection. • A new apta-sensing probe based on magnetic hydrophilic polymer (MHP) for detection of fetuin-A was proposed. • Arising from the synergistic effect of apatmer and polymer, the probe exhibited sensitive and selective detection for fetuin-A. • The probe was compatible with reported methods and could be applied in fetuin-A detection in serum.
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