Correlations between the dielectric properties and exterior morphology of cells revealed by dielectrophoretic field-flow fractionation.
Correlations between the dielectric properties and exterior morphology of cells revealed by dielectrophoretic field-flow fractionation.
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DOI：
10.1002/elps.201200496
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发表时间：
2013-04
期刊：
影响因子：
2.9
通讯作者：
Stemke-Hale, Katherine
中科院分区：
文献类型：
--
作者：
Gascoyne, Peter R. C.;Shim, Sangjo;Noshari, Jamileh;Becker, Frederick F.;Stemke-Hale, Katherine
关键词：
虽然介电电泳（DEP）有很大的潜力，解决临床细胞分离问题的基础上的细胞介电差异，预测DEP行为的细胞的生物学基础一直缺乏。在这里，NCI-60面板的肿瘤细胞类型的介电特性已被测量介电电泳（DEP）场流分离，与生长过程中的细胞的外部形态，并与外周血的亚群的介电和形态特征进行比较。与早期的研究结果一致，细胞总电容随细胞大小和质膜折叠而变化，悬浮液中的NCI-60细胞类型的介电性质反映了细胞在其生长部位的质膜面积和体积。因此，细胞在基于DEP的操作中的行为在很大程度上取决于它们在释放到悬浮液中之前的外部形态特征。因此，DEP能够区分具有不同形态起源的相似尺寸的细胞，例如，提供了优于基于尺寸的过滤的显著优势，用于分离循环肿瘤细胞。研究结果提供了一个框架，预测细胞介电行为的基础上的结构-功能关系，并建议DEP应广泛适用于作为一个表面标记的独立方法分选细胞。
Although dielectrophoresis (DEP) has great potential for addressing clinical cell isolation problems based on cell dielectric differences, a biological basis for predicting the DEP behavior of cells has been lacking. Here, the dielectric properties of the NCI-60 panel of tumor cell types have been measured by dielectrophoretic (DEP) field-flow fractionation, correlated with the exterior morphologies of the cells during growth, and compared with the dielectric and morphological characteristics of the subpopulations of peripheral blood. In agreement with earlier findings, cell total capacitance varied with both cell size and plasma membrane folding and the dielectric properties of the NCI-60 cell types in suspension reflected the plasma membrane area and volume of the cells at their growth sites. Therefore, the behavior of cells in DEP-based manipulations is largely determined by their exterior morphological characteristics prior to release into suspension. As a consequence, DEP is able to discriminate between cells of similar size having different morphological origins, offering a significant advantage over size-based filtering for isolating circulating tumor cells, for example. The findings provide a framework for anticipating cell dielectric behavior on the basis of structure-function relationships and suggest that DEP should be widely applicable as a surface marker-independent method for sorting cells.
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影响因子：
45.3
作者：
Cristofanilli, M;Hayes, DF;Terstappen, LWMM
通讯作者：
Terstappen, LWMM
DOI：
10.1073/pnas.92.3.860
发表时间：
1995-01-31
影响因子：
11.1
作者：
BECKER, FF;WANG, XB;GASCOYNE, PRC
通讯作者：
GASCOYNE, PRC
影响因子：
3
作者：
Chowdhury, H. H.;Zorec, R.
通讯作者：
Zorec, R.
影响因子：
7.4
作者：
Gascoyne, Peter R. C.
通讯作者：
Gascoyne, Peter R. C.
DOI：
10.1089/152581699319939
发表时间：
1999-10-01
期刊：
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
影响因子：
--
作者：
Huang, Y;Yang, J;Gascoyne, PRC
通讯作者：
Gascoyne, PRC