Role of sortase in Streptococcus mutans under the effect of nicotine.
Role of sortase in Streptococcus mutans under the effect of nicotine.
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DOI：
10.1038/ijos.2013.86
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发表时间：
2013-12
影响因子：
14.9
通讯作者：
Gregory, Richard L.
中科院分区：
文献类型：
--
作者：
Li, Ming-Yun;Huang, Rui-Jie;Zhou, Xue-Dong;Gregory, Richard L.
变形链球菌是一种常见的革兰氏阳性菌，在龋病中起重要作用。烟草和/或尼古丁对S.变形菌生长和定植。分选酶A被许多革兰氏阳性细菌使用，包括S。变形蛋白，以促进某些细胞表面蛋白的插入，含有LPXTGX基序，如抗原I/II。本研究探讨了尼古丁对分选酶A调控S.使用野生型S.变形杆菌NG 8及其同基因分选酶缺陷型和互补型菌株。简而言之，在生长、生物膜代谢和蔗糖诱导和唾液诱导的抗原I/II依赖性生物膜形成测定中，用增加量的尼古丁处理菌株。不同浓度的尼古丁对菌株的生长无显著影响。然而，随着尼古丁浓度的增加，它们的生物膜代谢活性显著增加（P≤0.05）（增加2至3倍）。此外，分选酶缺陷型菌株比野生型或分选酶补充型菌株在代谢上对尼古丁更敏感。由于尼古丁浓度的增加，所有菌株均显著增加蔗糖诱导的生物膜形成（增加2至3倍）。然而，分选酶缺陷菌株不能使尽可能多的蔗糖和唾液诱导的生物膜作为野生型NG 8没有增加尼古丁浓度。这些结果表明，尼古丁增加代谢活性和蔗糖诱导的生物膜形成。唾液诱导的生物膜形成测定和qPCR数据表明，抗原I/II用尼古丁上调，但生物膜不能像没有功能性分选酶A的野生型NG 8那样多地形成。
Streptococcus mutans is a common Gram-positive bacterium and plays a significant role in dental caries. Tobacco and/or nicotine have documented effects on S. mutans growth and colonization. Sortase A is used by many Gram-positive bacteria, including S. mutans, to facilitate the insertion of certain cell surface proteins, containing an LPXTGX motif such as antigen I/II. This study examined the effect of nicotine on the function of sortase A to control the physiology and growth of S. mutans using wild-type S. mutans NG8, and its isogenic sortase-defective and -complemented strains. Briefly, the strains were treated with increasing amounts of nicotine in planktonic growth, biofilm metabolism, and sucrose-induced and saliva-induced antigen I/II-dependent biofilm formation assays. The strains exhibited no significant differences with different concentrations of nicotine in planktonic growth assays. However, they had significantly increased (P≤0.05) biofilm metabolic activity (2- to 3-fold increase) as the concentration of nicotine increased. Furthermore, the sortase-defective strain was more sensitive metabolically to nicotine than the wild-type or sortase-complemented strains. All strains had significantly increased sucrose-induced biofilm formation (2- to 3-fold increase) as a result of increasing concentrations of nicotine. However, the sortase-defective strain was not able to make as much sucrose- and saliva-induced biofilm as the wild-type NG8 did with increasing nicotine concentrations. These results indicated that nicotine increased metabolic activity and sucrose-induced biofilm formation. The saliva-induced biofilm formation assay and qPCR data suggested that antigen I/II was upregulated with nicotine but biofilm was not able to be formed as much as wild-type NG8 without functional sortase A.
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DOI：
10.14219/jada.archive.1981.0372
发表时间：
1981-01-01
影响因子：
3.9
作者：
LINDEMEYER, RG;BAUM, RH;GOING, RE
通讯作者：
GOING, RE
DOI：
10.1034/j.1600-0528.2000.280408.x
发表时间：
2000-08-01
影响因子：
2.3
作者：
Milgrom, P;Riedy, CA;Bruss, J
通讯作者：
Bruss, J
影响因子：
4.9
作者：
Ramage, G;vande Walle, K;López-Ribot, JL
通讯作者：
López-Ribot, JL
影响因子：
2.2
作者：
ROEHM, NW;RODGERS, GH;GLASEBROOK, AL
通讯作者：
GLASEBROOK, AL
影响因子：
6
作者：
Schneider, NG;Jacob, P;Olmstead, RE
通讯作者：
Olmstead, RE