Quantitative Morphometry for Osteochondral Tissues Using Second Harmonic Generation Microscopy and Image Texture Information.
Quantitative Morphometry for Osteochondral Tissues Using Second Harmonic Generation Microscopy and Image Texture Information.
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DOI：
10.1038/s41598-018-21005-9
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发表时间：
2018-02-12
影响因子：
4.6
通讯作者：
Imamura T
中科院分区：
文献类型：
--
作者：
Saitou T;Kiyomatsu H;Imamura T
骨关节炎（OA）是一种慢性关节疾病，涉及关节软骨和软骨下骨的退变。我们之前建立了一种二次谐波生成（SHG）成像技术来评估OA小鼠模型中关节软骨的退行性改变。SHG成像是一种光学无标记技术，可以观察胶原原纤维，并表征关节胶原模式的关键变化。然而，如何量化组织胶原原纤维组织的形态学变化仍有待确定。在本研究中，我们采用基于纹理分析的方法解决了这一问题。探索利用灰度共生矩阵进行图像纹理分析，提取图像特征。我们研究了一种基于图像补丁的策略，该策略在原始图像的单个补丁上提取纹理特征，以捕获其中的局部结构模式。我们证实这种分析能够区分小鼠关节中的软骨组织和骨性组织。此外，我们将该方法应用于OA软骨病理评估，并观察到与使用现有特征描述符获得的结果相比，性能结果有所改善。所提出的方法可以应用于与胶原重塑和软骨和骨骼疾病相关的广泛条件。
Osteoarthritis (OA) is a chronic joint disorder involving degeneration of articular cartilage and subchondral bone in joints. We previously established a second harmonic generation (SHG) imaging technique for evaluating degenerative changes to articular cartilage in an OA mouse model. SHG imaging, an optical label-free technique, enabled observation of collagen fibrils, and characterized critical changes in the collagenous patterns of the joints. However, it still remains to be determined how morphological changes in the organization of tissue collagen fibrils should be quantified. In this study, we addressed this issue by employing an approach based on texture analysis. Image texture analysis using the gray level co-occurrence matrix was explored to extract image features. We investigated an image patch-based strategy, in which texture features were extracted on individual patches derived from original images to capture local structural patterns in them. We verified that this analysis enables discrimination of cartilaginous and osseous tissues in mouse joints. Moreover, we applied this method to OA cartilage pathology assessment, and observed improvements in the performance results compared with those obtained using an existing feature descriptor. The proposed approach can be applied to a wide range of conditions associated with collagen remodeling and diseases of cartilage and bone.
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