CD133: a potential indicator for differentiation and prognosis of human cholangiocarcinoma.
CD133: a potential indicator for differentiation and prognosis of human cholangiocarcinoma.
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DOI：
10.1186/1471-2407-11-320
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发表时间：
2011-07-29
期刊：
影响因子：
3.8
通讯作者：
Huang G
中科院分区：
文献类型：
--
作者：
Fan L;He F;Liu H;Zhu J;Liu Y;Yin Z;Wang L;Guo Y;Wang Z;Yan Q;Huang G
CD133 已知是癌症干细胞 (CSC) 标记物。然而，最近的研究表明，CD133不仅限于CSC，不仅在人类正常组织中表达，而且在某些癌症中表达，可以作为患者的预后因素。然而，CD133在人胆管癌（CC）中的表达很少，我们的研究是检测CD133在人CC中的表达并探索CD133的潜在功能。该研究纳入了 59 例病例，包括 5 份正常肝组织和 54 份连续 CC 标本（21 例高分化、12 例中分化和 21 例低分化）。采用CD133蛋白进行免疫组化染色，并进行统计分析。发现 CD133 在所有 5 个正常肝脏和 54 个具有不同亚细胞定位的 CC 组织中的 40 个 (74%) 中表达。在高分化、中分化和低分化病例中，CD133阳性病例数分别为19例（21例中19例，90%）、10例（12例中10例，83%）和11例（21例中11例，52%）。进一步统计分析表明CD133的表达及不同亚细胞定位与肿瘤的分化状态显着相关(P=0.004,P=0.009)。在 23 名生存随访患者中，14 名 CD133 阴性患者的中位生存期为 4 个月，而 9 名 CD133 阳性患者的中位生存期为 14 个月。在单变量生存分析中，CD133阴性表达与预后不良相关，而CD133阳性表达则预示着CC患者的良好预后（P = 0.001）。我们的研究表明CD133表达与CC的分化相关，并表明CD133是人类CC分化和预后的潜在指标。
CD133 is known to be a cancer stem cell (CSC) marker. However, recent studies have revealed that CD133 is not restricted to CSC but to be expressed not only in human normal tissues but also in some cancers and could serve as a prognostic factor for the patients. Nevertheless, the expression of CD133 in human cholangiocarcinoma (CC) is rare and our study is to detect the expression and explore the potential functions of CD133 in human CC. Fifty-nine cases, comprised of 5 normal liver tissues and 54 consecutive CC specimens (21 well-differentiated, 12 moderately-differentiated and 21 poorly-differentiated), were included in the study. Immunohistochemical stainning with CD133 protein was carried out, and statistical analyses were performed. CD133 was found to express in all 5 normal livers and 40 out of 54 (74%) CC tissues with different subcellular localization. In the well, moderately and poorly differentiated cases, the numbers of CD133 positive cases were 19 (19 of 21, 90%), 10 (10 of 12, 83%) and 11 (11 of 21, 52%) respectively. Further statistical analyses indicated that the expression and different subcellular localization of CD133 were significantly correlated with the differentiation status of tumors (P = 0.004, P = 0.009). Among 23 patients followed up for survival, the median survival was 4 months for fourteen CD133 negative patients but 14 months for nine CD133 positive ones. In univariate survival analysis, CD133 negative expression correlated with poor prognosis while CD133 positive expression predicted a favorable outcome of CC patients (P = 0.001). Our study demonstrates that CD133 expression correlates with the differentiation of CC and indicates that CD133 is a potential indicator for differentiation and prognosis of human CC.
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影响因子：
11.2
作者：
Collins, AT;Berry, PA;Maitland, NJ
通讯作者：
Maitland, NJ
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4
作者：
Marzesco, AM;Janich, P;Huttner, WB
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Huttner, WB
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15.9
作者：
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影响因子：
2.3
作者：
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影响因子：
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作者：
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