The validation of a functional, isolated pig bladder model for physiological experimentation.
The validation of a functional, isolated pig bladder model for physiological experimentation.
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DOI：
10.3389/fphar.2012.00052
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发表时间：
2012
影响因子：
5.6
通讯作者：
Vahabi B
中科院分区：
文献类型：
--
作者：
Parsons BA;Drake MJ;Gammie A;Fry CH;Vahabi B
描述膀胱的综合生理学需要整个器官的准备。这项研究的目的是通过动脉和膀胱内给药、膀胱内压力记录和表面微运动的拍摄来验证隔离的大型动物(猪)膀胱准备。母猪膀胱取自当地屠宰场，体外动脉灌流。记录不同容量的动脉压和膀胱内压。通过监测膀胱流入和流出的pH值来评估膀胱的存活率。动脉给药采用了引入灌流液中的丸剂。膀胱内给药包括缓慢的滴注和较长的停留时间。在测量膀胱内压力的同时，通过拍摄表面标记的分离来记录表面微运动。在长达8 h的时间内，所有膀胱层都获得了充分的灌流；流入和流出灌流液的pH值没有结构恶化或改变。动脉给药(卡巴胆碱和氯化钾)表现出一致的剂量依赖性反应。局部运动(微动)发生在膀胱表面，与膀胱内压的波动有不同的相关性。离体猪膀胱是研究整体膀胱生理的有效方法。它在体外灌流时仍然有效，对不同的给药途径有反应，并提供了一种模型，将膀胱壁的运动与膀胱内压的变化直接相关。
Characterizing the integrative physiology of the bladder requires whole organ preparations. The purpose of this study was to validate an isolated large animal (pig) bladder preparation, through arterial and intravesical drug administration, intravesical pressure recording, and filming of surface micromotions. Female pig bladders were obtained from the local abattoir and arterially perfused in vitro. Arterial and intravesical pressures were recorded at varying volumes. Bladder viability was assessed histologically and by monitoring inflow and outflow pH. Arterial drug administration employed boluses introduced into the perfusate. Intravesical administration involved slow instillation and a prolonged dwell-time. Surface micromotions were recorded by filming the separation of surface markers concurrently with intravesical pressure measurement. Adequate perfusion to all bladder layers was achieved for up to 8 h; there was no structural deterioration nor alteration in inflow and effluent perfusate pH. Arterial drug administration (carbachol and potassium chloride) showed consistent dose-dependent responses. Localized movements (micromotions) occurred over the bladder surface, with variable correlation with fluctuations of intravesical pressure. The isolated pig bladder is a valid approach to study integrative bladder physiology. It remains viable when perfused in vitro, responds to different routes of drug administration and provides a model to correlate movements of the bladder wall directly to variation of intravesical pressure.
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影响因子：
4.5
作者：
Drake, MJ;Harvey, IJ;van Duyl, WA
通讯作者：
van Duyl, WA
DOI：
10.1124/jpet.106.108902
发表时间：
2006-12-01
影响因子：
3.5
作者：
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通讯作者：
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影响因子：
6.6
作者：
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通讯作者：
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影响因子：
6.6
作者：
Mills, IW;Noble, JG;Brading, AF
通讯作者：
Brading, AF
影响因子：
6.6
作者：
CROWE, R;BURNSTOCK, G
通讯作者：
BURNSTOCK, G