Comparison of Immunogenicity Between a Candidate Live Attenuated Vaccine and an Inactivated Vaccine for Cache Valley Virus.
Comparison of Immunogenicity Between a Candidate Live Attenuated Vaccine and an Inactivated Vaccine for Cache Valley Virus.
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DOI：
10.1089/vim.2022.0103
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发表时间：
2023-01
期刊：
影响因子：
2.2
通讯作者：
Vanlandingham, Dana L.
中科院分区：
文献类型：
--
作者：
Ayers, Victoria B.;Huang, Yan-Jang S.;Kohl, Alain;Dunlop, James I.;Hettenbach, Susan M.;Park, So Lee;Higgs, Stephen;Vanlandingham, Dana L.
卡什谷病毒 (CVV) 是一种由蚊子传播的布尼亚病毒，在整个新世界流行。尽管 CVV 被认为是一种重要的农业病原体，主要与反刍动物胚胎致死和流产有关，但最近它已被认为是一种人畜共患病原体。随着对人类和兽医具有重要意义的布尼亚病毒的出现越来越多，人们在开发布尼亚病毒疫苗方面做出了巨大的努力。在这项研究中，对一种候选 CVV 减毒活疫苗 (LAV) 的免疫原性进行了评估，该疫苗含有非结构性小基因 (NSs) 和非结构性中型基因 (NSm) 的删除 (2delCVV)，并与通过使用二元乙烯亚胺 (BEI) 和氢氧化铝佐剂 (BEI-CVV) 灭活绵羊 CVV 而产生的自体候选疫苗进行了比较。 2delCVV和BEI-CVV均产生超过保护相关性的中和抗体反应，即空斑减少中和测试效价>10。然而，在初次免疫后第 63 天，2delCVV 比 BEI-CVV 更具免疫原性。这些结果保证了 2delCVV 作为候选 LAV 的进一步开发，并证明 NSs 和 NSm 基因的双重删除可以应用于疫苗的开发，并作为正布尼亚病毒的常见减毒策略。
Cache Valley virus (CVV) is a mosquito-borne bunyavirus that is enzootic throughout the new world. Although CVV is known as an important agricultural pathogen, primarily associated with embryonic lethality and abortions in ruminants, it has recently been recognized for its expansion as a zoonotic pathogen. With the increased emergence of bunyaviruses with human and veterinary importance, there have been significant efforts dedicated to the development of bunyavirus vaccines. In this study, the immunogenicity of a candidate live-attenuated vaccine (LAV) for CVV, which contains the deletion of the nonstructural small (NSs) and nonstructural medium (NSm) genes (2delCVV), was evaluated and compared with an autogenous candidate vaccine created through the inactivation of CVV using binary ethylenimine (BEI) with an aluminum hydroxide adjuvant (BEI-CVV) in sheep. Both 2delCVV and BEI-CVV produced a neutralizing antibody response that exceeds the correlate of protection, that is, plaque reduction neutralization test titer >10. However, on day 63 postinitial immunization, 2delCVV was more immunogenic than BEI-CVV. These results warrant further development of 2delCVV as a candidate LAV and demonstrate that the double deletion of the NSs and NSm genes can be applied to the development of vaccines and as a common attenuation strategy for orthobunyaviruses.
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