A new phase model of the spatiotemporal relationships between three circadian oscillators in the brainstem.
A new phase model of the spatiotemporal relationships between three circadian oscillators in the brainstem.
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DOI：
10.1038/s41598-023-32315-y
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发表时间：
2023-04-04
影响因子：
4.6
通讯作者：
Piggins, Hugh D.
中科院分区：
文献类型：
--
作者：
Ahern, Jake;Chrobok, Lukasz;Champneys, Alan R.;Piggins, Hugh D.
对先前在小鼠背侧迷走神经复合体中记录的PER2生物发光节律的分析揭示了三个不同的昼夜节律振荡器之间的特征相位关系。这些信号代表核心时钟基因在最后区(AP)、孤束核(NTS)和第四脑室周围的室管膜细胞(4Vep)中的表达。最初，数据表明有一个一致的阶段，AP首先达到峰值，紧随其后的是NTS，4VEP在8-9小时后达到峰值。小波分析表明，这种模式并不是在整个记录过程中保持一致的，然而，相位动力学强烈地表明振子相互作用的存在。建立了一个简单的三个振荡器的相模型，它表明三个模型振荡器之间存在真实的相动力学，并且耦合接近同步转变。耦合拓扑结构表明，AP向NTS和4VEP双向传递相位信息，以同步这三个结构。将该模型与以往的实验结果进行了比较，验证了该模型解释DVC昼夜节律变化的可行性。最后，我们证明了模拟稳定衰减的耦合提高了模型捕捉实验相动力学的能力。
Analysis of ex vivo Per2 bioluminescent rhythm previously recorded in the mouse dorsal vagal complex reveals a characteristic phase relationship between three distinct circadian oscillators. These signals represent core clock gene expression in the area postrema (AP), the nucleus of the solitary tract (NTS) and the ependymal cells surrounding the 4th ventricle (4Vep). Initially, the data suggests a consistent phasing in which the AP peaks first, followed shortly by the NTS, with the 4Vep peaking 8–9 h later. Wavelet analysis reveals that this pattern is not consistently maintained throughout a recording, however, the phase dynamics strongly imply that oscillator interactions are present. A simple phase model of the three oscillators is developed and it suggests that realistic phase dynamics occur between three model oscillators with coupling close to a synchronisation transition. The coupling topology suggests that the AP bidirectionally communicates phase information to the NTS and the 4Vep to synchronise the three structures. A comparison of the model with previous experimental manipulations demonstrates its feasibility to explain DVC circadian phasing. Finally, we show that simulating steadily decaying coupling improves the model’s ability to capture experimental phase dynamics.
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