Peripheral blood circular RNA hsa_circ_0124644 can be used as a diagnostic biomarker of coronary artery disease.
Peripheral blood circular RNA hsa_circ_0124644 can be used as a diagnostic biomarker of coronary artery disease.
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DOI：
10.1038/srep39918
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发表时间：
2017-01-03
影响因子：
4.6
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Zhao Z;Li X;Gao C;Jian D;Hao P;Rao L;Li M
本研究的目的是探讨冠状动脉疾病（CAD）患者外周血中环状RNA（circRNA）的表达及其作为CAD诊断生物标志物的潜在用途。我们首先通过RNA微阵列分析了12名CAD患者和12名对照个体的外周血circRNA，发现这两组之间有22个circRNA差异表达：12个上调，10个下调。然后，我们在更严格的筛选标准下选择了5种circRNA作为候选生物标志物，并在另一组由30名对照个体和30名具有不同SYNTAX评分的CAD患者组成的受试者中进行验证。这5种circRNA在CAD组中均显著增加。Hsa_circ_0124644的曲线下面积（AUC）最大。我们在由115名对照个体和137名CAD患者组成的独立队列中测试了hsa_circ_0124644。纳入CAD危险因素后，AUC从0.769（95%置信区间= [0.710-0.827]，P < 0.001）略微增加至0.804（[0.751-0.857]，P < 0.001），与hsa_circ_0098964联合使用时，诊断价值略微增加。综上所述，我们的结果表明，hsa_circ_0124644可以用作CAD的诊断生物标志物。
The aim of the present study was to investigate the expression of circular RNAs (circRNAs) in the peripheral blood of coronary artery disease (CAD) patients and the potential use of circRNAs as diagnostic biomarkers of CAD. We first analysed peripheral blood circRNAs of 12 CAD patients and 12 control individuals by RNA microarray and found that 22 circRNAs were differentially expressed between these two groups: 12 were upregulated, and 10 were downregulated. Then, we selected 5 circRNAs as candidate biomarkers under stricter screening criteria and verified them in another group of subjects consisting of 30 control individuals and 30 CAD patients with different SYNTAX scores. These 5 circRNAs were all remarkably increased in the CAD group. Hsa_circ_0124644 had the largest area under the curve (AUC). We tested hsa_circ_0124644 in an independent cohort consisting of 115 control individuals and 137 CAD patients. After we included the risk factors for CAD, the AUC slightly increased from 0.769 (95% confidence interval = [0.710–0.827], P < 0.001) to 0.804 ([0.751–0.857], P < 0.001), and when combined with hsa_circ_0098964, the diagnostic value slightly increased. Taken together, our results suggest that hsa_circ_0124644 can be used as a diagnostic biomarker of CAD.
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影响因子：
4.5
作者：
Burd CE;Jeck WR;Liu Y;Sanoff HK;Wang Z;Sharpless NE
通讯作者：
Sharpless NE
影响因子：
2.1
作者：
Gidlöf O;Smith JG;Miyazu K;Gilje P;Spencer A;Blomquist S;Erlinge D
通讯作者：
Erlinge D
影响因子：
--
作者：
Li F;Zhang L;Li W;Deng J;Zheng J;An M;Lu J;Zhou Y
通讯作者：
Zhou Y
影响因子：
4.6
作者：
Bachmayr-Heyda A;Reiner AT;Auer K;Sukhbaatar N;Aust S;Bachleitner-Hofmann T;Mesteri I;Grunt TW;Zeillinger R;Pils D
通讯作者：
Pils D
影响因子：
2.2
作者：
Jiang, Yue;Tian, Jun-Ping;Du, Feng-He
通讯作者：
Du, Feng-He