Phylogenetic and pathogenic characterization of a pigeon paramyxovirus type 1 isolate reveals cross-species transmission and potential outbreak risks in the northwest region of China
Phylogenetic and pathogenic characterization of a pigeon paramyxovirus type 1 isolate reveals cross-species transmission and potential outbreak risks in the northwest region of China
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鸽子副粘病毒 1 型分离株的系统发育和致病特征揭示了中国西北地区的跨物种传播和潜在的暴发风险
DOI：
10.1007/s00705-017-3422-1
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发表时间：
2017-06
影响因子：
2.7
通讯作者：
Yang Zengqi
中科院分区：
文献类型：
--
作者：
Ren Shanhui;Wang Chongyang;Zhang Xue;Zhao Lei;Wang Xiangwei;Yao Wei;Han Qingsong;Wang Yanhong;Fan Mengfei;Gao Xiaolong;Xiao Sa;Wang Xinglong;Yang Zengqi
鸽副粘病毒1型(ppmv-1)在鸽子中流行，给许多国家的养殖业造成了严重的经济损失。然而，ppmv-1传播的确切流行过程仍不清楚。在本研究中，我们分析了ppmv-1/SX-01/15分离株的全基因组。测序结果表明，该病毒基因组全长15,192个核苷酸，基因顺序为3‘-NP-P-M-F-HN-L-5’。系统进化分析表明，该毒株属于亚型Vic，平均死亡时间(MDT)为62.4h，脑内致病指数(ICPI)为1.13，为中源Pmv-1毒株。据我们所知，这是在中国西北地区商业鸽群中流行的亚型Vic中基因ppmv-1毒株的首次报道。在比较感染实验中，4周龄鸽子的发病率和死亡率分别为100%和80%，而5周龄雏鸡的发病率和死亡率分别为50%和30%。此外，这种病毒导致4周大的鸽子出现严重的神经症状，5周大的鸡出现轻微的神经症状。脑组织病理学检查显示为典型的非化脓性脑炎病变。病毒的脱落模式、病毒载量和病毒分布在感染鸡和鸽子之间是不同的。基因组特征表明，至少从2006年到2015年，该地区存在ppmv-1亚型Vic的跨物种传播。
Pigeon paramyxovirus type-1 (PPMV-1) is enzootic in pigeons, causing severe economic loss in the poultry industry in many countries. However, the exact epidemic process of PPMV-1 transmission is still unclear. In this study, we analyzed the complete genome of the PPMV-1/SX-01/15 isolate. Sequence results show that the virus genome contains 15,192 nucleotides, with the gene order 3’-NP-P-M-F-HN-L-5’. Phylogenetic analysis revealed that this genome belongs to subgenotype VIc in class II. The mean death time (MDT) and intracerebral pathogenicity index (ICPI) were 62.4 h and 1.13, respectively, indicating that this isolate is a mesogenic PPMV-1 strain. To our knowledge, this is the first report of a subgenotype VIc mesogenic PPMV-1 strain circulating in commercial pigeon flocks in the northwest region of China. In a comparative infection experiment, the morbidity and mortality rates were 100% and 80%, respectively, in 4-week-old pigeons, whereas they were 50% and 30%, respectively, in 5-week-old chickens. Furthermore, this virus caused severe neurological symptoms in a 4-week-old pigeon and mild neurological symptoms in a 5-week-old chicken. A histopathological examination of the brain showed a classical nonsuppurative encephalitis lesion. The pattern of viral shedding, and viral load, and virus distribution differed between infected chickens and pigeons. Genomic characteristics suggest that there was cross-species transmission of PPMV-1 subgenotype VIc in this region at least from the years 2006 to 2015.
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影响因子：
3.8
作者：
Dortmans, J. C. F. M.;Rottier, P. J. M.;Peeters, B. P. H.
通讯作者：
Peeters, B. P. H.
影响因子：
4.4
作者：
Garcia, Stivalis Cardenas;Lopez, Roberto Navarro;Afonso, Claudio L.
通讯作者：
Afonso, Claudio L.
中国朱鹮(Nipponia nippon)分离的两种强效新城疫病毒的系统发育和病原学分析
影响因子：
1.6
作者：
Chen, Shengli;Hao, Huafang;Yang, Zengqi
通讯作者：
Yang, Zengqi
影响因子：
2.7
作者：
COLLINS, MS;ALEXANDER, DJ;MANVELL, RJ
通讯作者：
MANVELL, RJ
影响因子：
5.4
作者：
IORIO, RM;SYDDALL, RJ;RIEL, AM
通讯作者：
RIEL, AM