Nanofibrous ε-polycaprolactone scaffolds containing Ag-doped magnetite nanoparticles: Physicochemical characterization and biological testing for wound dressing applications in vitro and in vivo.
Nanofibrous ε-polycaprolactone scaffolds containing Ag-doped magnetite nanoparticles: Physicochemical characterization and biological testing for wound dressing applications in vitro and in vivo.
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DOI：
10.1016/j.bioactmat.2020.12.026
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发表时间：
2021-07
影响因子：
18.9
通讯作者：
Uskoković V
中科院分区：
文献类型：
--
作者：
Ahmed MK;Zayed MA;El-Dek SI;Hady MA;El Sherbiny DH;Uskoković V
皮肤伤口可导致许多并发症，对健康造成危险后果。在这项工作中，磁性纳米颗粒掺杂不同浓度的抗菌银（Ag）离子，并将其纳入电纺丝纳米纤维ε-聚己内酯（PCL）支架中。采用一系列物理化学技术对不同银含量的纳米颗粒和支架进行了表征。银作为阳离子进入磁铁矿，并优先定位在四面体位置，导致晶格畸变和地形不规则。由于银的容纳，结构的非晶化扩大了体中的晶格，并在表面上缩小了晶格，在表面上发现了Fe-O配位分布的扩大。Ag通过改变铁离子和亚铁离子的分布，促进自旋倾斜，减少双交换相互作用，使磁铁矿的磁性软化。Ag修饰了纳米颗粒与聚合物的界面，促进了纳米颗粒从聚合物纳米纤维表面的突出，从而增加了聚合物纳米纤维的粗糙度和亲水性，对细胞的粘附和生长产生了积极的影响。人黑素细胞活力及对大肠杆菌和金黄色葡萄球菌的抑菌活性均随银在磁铁矿相浓度的增加而增加。大鼠皮肤创面愈合率也与磁铁矿期银浓度成正比增加，治疗组第10天真皮和表皮组织未见异常。这些结果意味着这些复合纳米纤维支架在伤口敷料和其他重建皮肤治疗方面具有良好的潜力。制备了掺杂银离子的磁铁矿纳米粒子静电纺纳米纤维聚合物敷料。详细的物理化学特性提供了借助衍射，光谱和显微技术。随着银离子的加入，黑素细胞的活力和抗菌活性均有所提高。与对照组相比，不含银和含银敷料的大鼠皮肤创面愈合率在第10天分别提高到51%和92%。用掺银敷料处理的动物没有出现对照组动物皮脂腺萎缩和毛囊坏死。
Skin wounds can lead to numerous complications with dangerous health consequences. In this work, magnetite nanoparticles were doped with different concentrations of antimicrobial silver (Ag) ions and incorporated into the electrospun nanofibrous ε-polycaprolactone (PCL) scaffolds. Nanoparticles and scaffolds with various Ag contents were characterized using a range of physicochemical techniques. Ag entered magnetite as cations and preferentially positioned at tetrahedral sites, introducing lattice distortions and topographic irregularities. Amorphization of the structure due to accommodation of Ag expanded the lattice in the bulk and contracted it on the surface, where broadened distribution of Fe–O coordinations was detected. Promoting spin canting and diminishing the double exchange interaction through altered distribution of ferric and ferrous ions, Ag softened the magnetism of magnetite. By making the nanoparticle structure more defective, Ag modified the interface with the polymer and promoted the protrusion of the nanoparticles from the surface of the polymeric nanofibers, thus increasing their roughness and hydrophilicity, with positive repercussions on cell adhesion and growth. Both the viability of human melanocytes and the antibacterial activity against E. coli and S. aureus increased with the concentration of Ag in the magnetite phase of the scaffolds. Skin wound healing rate in rats also increased in direct proportion with the concentration of Ag in the magnetite phase, and no abnormalities in the dermal and epidermal tissues were visible on day 10 in the treatment group. These results imply an excellent potential of these composite nanofibrous scaffolds for use as wound dressings and in other reconstructive skin therapies. Electrospun nanofibrous polymeric wound dressings interspersed with magnetite nanoparticles doped with Ag ions were fabricated. Detailed physicochemical characterization is provided with aid of diffractometric, spectroscopic and microscopic techniques. Both the viability of melanocytes and the antibacterial activity increased with the addition of Ag ions. Skin wound healing rate in rats increased to 51 and 92 % on day 10 for dressings without and with Ag, respectively, relative to control. Animals treated with Ag-doped dressings displayed no atrophy of sebaceous glands and necrosis of hair follicles of control animals.
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DOI：
10.1016/j.pnsc.2017.06.004
发表时间：
2017-08-01
影响因子：
4.7
作者：
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