Slow moving neural source in the epileptic hippocampus can mimic progression of human seizures.
Slow moving neural source in the epileptic hippocampus can mimic progression of human seizures.
复制标题
癫痫海马体中缓慢移动的神经源可以模拟人类癫痫发作的进展
DOI：
10.1038/s41598-018-19925-7
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发表时间：
2018-01-24
影响因子：
4.6
通讯作者：
Durand DM
中科院分区：
文献类型：
--
作者：
Chiang CC;Wei X;Ananthakrishnan AK;Shivacharan RS;Gonzalez-Reyes LE;Zhang M;Durand DM
已经观察到快速和慢速神经波在癫痫发作期间在人脑中传播。然而，这些波的性质很难在手术环境中研究。在这里，我们报告了两个不同的行波在体外癫痫海马体中传播的速度类似于在人脑中的观察。用基因编码的电压敏感荧光蛋白（VSFP Butterfly 1.2）和高速摄像机同时记录快速行进的尖峰和缓慢移动的波。研究结果表明，快行锋电位对NMDA敏感，慢行波对NMDA不敏感。图像分析和模型模拟表明，慢波移动缓慢，产生快速行进的棘波，因此是癫痫样活动的移动源。这种缓慢移动的波与用钙染料（OGB-1）检测到的传播的神经钙波相关，但不依赖于NMDA受体，与ATP释放无关，并且比先前记录的钾波快得多。计算机模拟表明，缓慢移动的波可以传播的ephaptic效应，如癫痫样活动。这些发现为与快速传播尖峰相关的缓慢传播癫痫发作波前提供了另一种解释。
Fast and slow neural waves have been observed to propagate in the human brain during seizures. Yet the nature of these waves is difficult to study in a surgical setting. Here, we report an observation of two different traveling waves propagating in the in-vitro epileptic hippocampus at speeds similar to those in the human brain. A fast traveling spike and a slow moving wave were recorded simultaneously with a genetically encoded voltage sensitive fluorescent protein (VSFP Butterfly 1.2) and a high speed camera. The results of this study indicate that the fast traveling spike is NMDA-sensitive but the slow moving wave is not. Image analysis and model simulation demonstrate that the slow moving wave is moving slowly, generating the fast traveling spike and is, therefore, a moving source of the epileptiform activity. This slow moving wave is associated with a propagating neural calcium wave detected with calcium dye (OGB-1) but is independent of NMDA receptors, not related to ATP release, and much faster than those previously recorded potassium waves. Computer modeling suggests that the slow moving wave can propagate by the ephaptic effect like epileptiform activity. These findings provide an alternative explanation for slow propagation seizure wavefronts associated with fast propagating spikes.
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影响因子：
16.6
作者：
Martinet LE;Fiddyment G;Madsen JR;Eskandar EN;Truccolo W;Eden UT;Cash SS;Kramer MA
通讯作者：
Kramer MA
影响因子：
6.1
作者：
Derchansky, M.;Rokni, D.;Carlen, P. L.
通讯作者：
Carlen, P. L.
影响因子：
2.5
作者：
Golomb, David;Yue, Cuiyong;Yaari, Yoel
通讯作者：
Yaari, Yoel
影响因子：
2.5
作者：
KONNERTH, A;HEINEMANN, U;YAARI, Y
通讯作者：
YAARI, Y
影响因子：
16.2
作者：
Grienberger, Christine;Konnerth, Arthur
通讯作者：
Konnerth, Arthur