Characterization of aid1, a novel gene involved in Fusobacterium nucleatum interspecies interactions.
Characterization of aid1, a novel gene involved in Fusobacterium nucleatum interspecies interactions.
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DOI：
10.1007/s00248-014-0400-y
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发表时间：
2014-08
影响因子：
3.6
通讯作者：
Lux, Renate
中科院分区：
文献类型：
--
作者：
Kaplan, Aida;Kaplan, Christopher W.;He, Xuesong;McHardy, Ian;Shi, Wenyuan;Lux, Renate
已知口腔条件致病菌核梭杆菌与居住在口腔内的大量不同细菌相互作用。它通过精氨酸抑制粘附素RadD粘附在多种革兰氏阳性细菌上，包括口服链球菌。在这项研究中，我们描述了一种由预测的开放阅读框FN1253编码的新蛋白，该蛋白似乎在具核梭菌的种间相互作用中发挥作用，特别是与口腔链球菌和相关革兰氏阳性菌的相互作用。我们指定FN1253为aid1（粘附诱导决定因子1）。表达分析表明，该基因在具核梭菌单一物种生物膜中被诱导，而已知粘附于具核梭菌的口腔微生物群的代表性成员的存在触发了其抑制。aid1的失活和过表达影响了具核梭菌与口腔链球菌和密切相关的粪肠球菌共聚集的能力，但对其他革兰氏阳性口腔菌没有影响。此外，aid1的过表达导致具核梭菌与口腔链球菌的双种生物膜结构发生剧烈变化。Aid1功能在精氨酸的存在下被废除，并被发现依赖于RadD。有趣的是，aid1的差异表达并不影响RadD的mRNA和蛋白水平。这些发现表明，radd介导的口腔链球菌的黏附涉及比黏附素在伴侣菌株表面的简单相互作用更复杂的细胞过程。Aid1可能通过增加具核梭菌与其他微生物的结合特异性，在促进radd介导的与口腔链球菌的相互作用中发挥重要作用。
The oral opportunistic pathogen Fusobacterium nucleatum is known to interact with a large number of different bacterial species residing in the oral cavity. It adheres to a variety of Gram-positive bacteria, including oral streptococci via the arginine-inhibitable adhesin RadD. In this study, we describe a novel protein encoded by the predicted open reading frame FN1253 that appears to play a role in interspecies interactions of F. nucleatum, particularly with oral streptococci and related Gram-positive species. We designated FN1253 as aid1 (Adherence Inducing Determinant 1). Expression analyses demonstrated that this gene was induced in F. nucleatum single species biofilms, while the presence of representative members of the oral microbiota known to adhere to F. nucleatum triggered its suppression. Inactivation as well as overexpression of aid1 affected the ability of F. nucleatum to coaggregate with oral streptococci and the closely related Enterococcus faecalis, but not other Gram-positive oral species tested. Furthermore, overexpression of aid1 led to a drastic change in the structure of dual species biofilms of F. nucleatum with oral streptococci. Aid1 function was abolished in the presence of arginine and found to be dependent on RadD. Interestingly, differential expression of aid1 did not affect mRNA and protein levels of RadD. These findings indicate that RadD-mediated adhesion to oral streptococci involves more complex cellular processes than the simple interaction of adhesins on the surface of partner strains. Aid1 could potentially play an important role in facilitating RadD-mediated interaction with oral streptococci by increasing binding specificity of F. nucleatum to other microbial species.
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影响因子：
4.4
作者：
Hsiao WW;Li KL;Liu Z;Jones C;Fraser-Liggett CM;Fouad AF
通讯作者：
Fouad AF
DOI：
10.1016/j.jalz.2011.04.006
发表时间：
2012-05
期刊：
Alzheimer's & dementia : the journal of the Alzheimer's Association
影响因子：
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作者：
Sparks Stein P;Steffen MJ;Smith C;Jicha G;Ebersole JL;Abner E;Dawson D 3rd
通讯作者：
Dawson D 3rd
影响因子：
3.2
作者：
Haake, SK;Yoder, SC;Podkaminer, K
通讯作者：
Podkaminer, K
影响因子：
3.1
作者：
OHTA, K;MAKINEN, KK;LOESCHE, WJ
通讯作者：
LOESCHE, WJ
DOI：
10.1073/pnas.0505454102
发表时间：
2005-11-08
影响因子：
11.1
作者：
Kim, SY;Kim, MS;Chun, MM
通讯作者：
Chun, MM