A tunable synthetic hydrogel system for culture of retinal ganglion cells and amacrine cells.
A tunable synthetic hydrogel system for culture of retinal ganglion cells and amacrine cells.
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DOI：
10.1016/j.actbio.2013.04.048
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发表时间：
2013-08
影响因子：
9.7
通讯作者：
Goldberg, Jeffrey L.
中科院分区：
文献类型：
--
作者：
Hertz, Jonathan;Robinson, Rebecca;Valenzuela, Daniel A.;Layik, Erin B.;Goldberg, Jeffrey L.
中枢神经系统（CNS）由复杂的单个细胞群组成，这些细胞从其局部环境接收电，化学和物理信号。标准的体外细胞培养方法依赖于二维（2D）基底，其很难模拟体内神经结构。在三维（3D）培养系统中生长的神经细胞可以提供一个机会，研究比2D培养更准确的体内环境的代表。此外，每种特定类型的神经元取决于其局部环境的离散组成和物理特性。以前，我们开发了一个库的水凝胶组成的聚（乙二醇）（PEG）和聚（L-赖氨酸）（PLL），表现出广泛的机械性能。在这里，我们确定了特定的支架从这个库，很容易支持纯化的视网膜神经节细胞（RGC）和无长突细胞（AC）的生存，迁移和神经突生长。这些数据解决了关于神经元与其局部环境的物理和化学性质相互作用的重要生物学问题，并为损伤后的细胞替代疗法提供了工程神经组织的进一步见解。
The central nervous system (CNS) consists of complex groups of individual cells that receive electrical, chemical, and physical signals from their local environment. Standard in vitro cell culture methods rely on two-dimensional (2D) substrates that poorly simulate in vivo neural architecture. Neural cells grown in three-dimensional (3D) culture systems may provide an opportunity to study more accurate representations of the in vivo environment than 2D cultures. Furthermore, each specific type of neuron depends on discrete compositions and physical properties of their local environment. Previously, we developed a library of hydrogels composed of poly(ethylene glycol) (PEG) and poly(L-lysine) (PLL) which exhibit a wide range of mechanical properties. Here, we identified specific scaffolds from this library that readily support the survival, migration and neurite outgrowth of purified retinal ganglion cells (RGCs) and amacrine cells (ACs). These data address important biological questions about the interaction of neurons with the physical and chemical properties of their local environment and provide further insight for engineering neural tissue for cell-replacement therapies following injury.
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影响因子：
3.8
作者：
Tibbitt, Mark W.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
影响因子：
14
作者：
Kador, Karl E.;Montero, Ramon B.;Venugopalan, Praseeda;Hertz, Jonathan;Zindell, Allison N.;Valenzuela, Daniel A.;Uddin, Mohammed S.;Lavik, Erin B.;Muller, Kenneth J.;Andreopoulos, Fotios M.;Goldberg, Jeffrey L.
通讯作者：
Goldberg, Jeffrey L.
影响因子：
3.4
作者：
Nakatani, Masayoshi;Shinohara, Yuko;Nitta, Atsumi
通讯作者：
Nitta, Atsumi
影响因子：
2.9
作者：
KLEINMAN, HK;MCGARVEY, ML;MARTIN, GR
通讯作者：
MARTIN, GR
DOI：
10.1023/a:1008915305681
发表时间：
1998-12-01
影响因子：
3.7
作者：
Rainaldi, G;Calcabrini, A;Santini, MT
通讯作者：
Santini, MT