Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): Prevalence and genetic diversity of Blastocystis subtypes.
Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): Prevalence and genetic diversity of Blastocystis subtypes.
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DOI：
10.1371/journal.pone.0193860
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Santos HLC
中科院分区：
文献类型：
--
作者：
Barbosa CV;Barreto MM;Andrade RJ;Sodré F;d'Avila-Levy CM;Peralta JM;Igreja RP;de Macedo HW;Santos HLC
肠道寄生虫感染被认为是一个严重的公共卫生问题，在世界范围内广泛分布，主要分布在热带和亚热带国家的城市和农村环境中。在全球范围内，土壤传播的蠕虫和原生动物是最常见的肠道寄生虫。芽囊原虫（Blastocystis sp.）是一种高度流行的疑似致病性原生动物，由于其显著的遗传多样性和宿主可塑性而被认为是一种不寻常的原生生物。从巴西里约热内卢三个农村山谷的居民中收集了294份粪便样本。粪便标本采用寄生虫学方法、粪便培养、巢式PCR和PCR/测序进行评价。通过寄生虫学分析，总体患病率为64.3%（294例病例中的189例）。芽囊藻其次是微小内毒素血症（Endolimax nana溶组织内阿米巴复合群18.7%钩虫感染（7.1%），大肠内阿米巴（5.8%），贾第鞭毛虫布氏碘阿米巴（4.1%）（1.0%），鞭虫感染率为1.0%、人五毛滴虫0.7%、蛲虫0.7%、蛔虫0.7%、粪类圆线虫0.7%。IPIs的患病率在性别上存在显著差异。Blastocystis sp.的系统发育分析和BLAST检索显示了5种不同的亚型：ST 3（34.0%），ST 1（27.0%），ST 2（27.0%），ST 4（3.5%），ST 8（7.0%）和一个未鉴定的亚型。我们的研究结果表明，巴西里约热内卢Sumidouro市农村地区的肠道寄生虫感染率仍然很高，仍然是对公共卫生的挑战。此外，我们的数据揭示了芽囊原虫亚型和可能的新亚型的显着遗传异质性，其确认将需要更多的数据。我们的研究有助于了解潜在的传播途径，流行病学和遗传多样性的芽囊原虫在农村地区在区域和全球范围内。
Intestinal parasitic infections are considered a serious public health problem and widely distributed worldwide, mainly in urban and rural environments of tropical and subtropical countries. Globally, soil-transmitted helminths and protozoa are the most common intestinal parasites. Blastocystis sp. is a highly prevalent suspected pathogenic protozoan, and considered an unusual protist due to its significant genetic diversity and host plasticity. A total of 294 stool samples were collected from inhabitants of three rural valleys in Rio de Janeiro, Brazil. The stool samples were evaluated by parasitological methods, fecal culture, nested PCR and PCR/Sequencing. Overall prevalence by parasitological analyses was 64.3% (189 out of 294 cases). Blastocystis sp. (55.8%) was the most prevalent, followed by Endolimax nana (18.7%), Entamoeba histolytica complex (7.1%), hookworm infection (7.1%), Entomoeba coli (5.8%), Giardia intestinalis (4.1%), Iodamoeba butchilii (1.0%), Trichuris trichiura (1.0%), Pentatrichomonas hominis (0.7%), Enterobius vermicularis (0.7%), Ascaris lumbricoides (0.7%) and Strongyloides stercoralis (0.7%). Prevalence of IPIs was significantly different by gender. Phylogenetic analysis of Blastocystis sp. and BLAST search revealed five different subtypes: ST3 (34.0%), ST1 (27.0%), ST2 (27.0%), ST4 (3.5%), ST8 (7.0%) and a non-identified subtype. Our findings demonstrate that intestinal parasite infection rates in rural areas of the Sumidouro municipality of Rio de Janeiro, Brazil are still high and remain a challenge to public health. Moreover, our data reveals significant genetic heterogeneity of Blastocystis sp. subtypes and a possible novel subtype, whose confirmation will require additional data. Our study contributes to the understanding of potential routes of transmission, epidemiology, and genetic diversity of Blastocystis sp. in rural areas both at a regional and global scale.
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影响因子：
3.2
作者：
David ÉB;Guimarães S;de Oliveira AP;Goulart de Oliveira-Sequeira TC;Nogueira Bittencourt G;Moraes Nardi AR;Martins Ribolla PE;Bueno Franco RM;Branco N;Tosini F;Bella A;Pozio E;Cacciò SM
通讯作者：
Cacciò SM
影响因子：
2
作者：
Balint, Anita;Doczi, Ilona;Molnar, Tamas
通讯作者：
Molnar, Tamas
影响因子：
1.5
作者：
Clark, CG
通讯作者：
Clark, CG
影响因子：
--
作者：
Boonjaraspinyo, Sirintip;Boonmars, Thidarut;Sriraj, Pranee
通讯作者：
Sriraj, Pranee
影响因子：
2
作者：
Helenbrook, William D.;Shields, William M.;Whipps, Christopher M.
通讯作者：
Whipps, Christopher M.