Directed microtubule growth, +TIPs, and kinesin-2 are required for uniform microtubule polarity in dendrites.
Directed microtubule growth, +TIPs, and kinesin-2 are required for uniform microtubule polarity in dendrites.
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DOI：
10.1016/j.cub.2010.11.050
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发表时间：
2010-12-21
期刊：
影响因子：
9.2
通讯作者：
Rolls, Melissa M.
中科院分区：
文献类型：
--
作者：
Mattie, Floyd J.;Stackpole, Megan M.;Stone, Michelle C.;Clippard, Jessie R.;Rudnick, David A.;Qiu, Yijun;Tao, Juan;Allender, Dana L.;Parmar, Manpreet;Rolls, Melissa M.
在许多分化的细胞中，微管被组织成极化的非中心体阵列，但很少有控制这些阵列的机制已被确定。例如，机制，保持微管极性在面对不断重塑的动态不稳定性是未知的。果蝇神经元在树突中含有极性一致的负端微管，这些微管通常高度分支。由于通过树突分支点的无向微管生长会危及微管的均匀极性，因此我们使用该系统来了解细胞如何维持极化微管的动态阵列。我们发现，生长中的微管通过相同的方式转向树突分支点，朝向细胞体，98%的时间，并且生长中的微管沿着沿着稳定的微管朝向它们的正端。使用RNAi和遗传方法，我们表明，驱动蛋白-2，+TIPS EB 1和APC，所需的均匀树突微管极性。此外，Apc 2-GFP和Apc-RFP的蛋白质-蛋白质相互作用和定位到分支点表明这些蛋白质在树突分支处一起工作。驱动蛋白-2减少的神经元无法从树突再生轴突，证明了这种极性机制的功能重要性。我们的结论是，微管生长是针对树突分支点，驱动蛋白-2，APC和EB 1可能在这个过程中发挥作用。我们建议，被招募到增长的微管+TIPS，和马达蛋白转向增长的微管在分支点。这代表了一种新发现的维持微管极化阵列的机制。
In many differentiated cells microtubules are organized into polarized noncentrosomal arrays, yet few mechanisms that control these arrays have been identified. For example, mechanisms that maintain microtubule polarity in the face of constant remodeling by dynamic instability are not known. Drosophila neurons contain uniform polarity minus-end-out microtubules in dendrites, which are often highly branched. As undirected microtubule growth through dendrite branch points jeopardizes uniform microtubule polarity, we have used this system to understand how cells can maintain dynamic arrays of polarized microtubules. We find that growing microtubules navigate dendrite branch points by turning the same way, towards the cell body, 98% of the time, and that growing microtubules track along stable microtubules towards their plus ends. Using RNAi and genetic approaches, we show that kinesin-2, and the +TIPS EB1 and APC, are required for uniform dendrite microtubule polarity. Moreover, the protein-protein interactions and localization of Apc2-GFP and Apc-RFP to branch points suggests these proteins work together at dendrite branches. The functional importance of this polarity mechanism is demonstrated by the failure of neurons with reduced kinesin-2 to regenerate an axon from a dendrite. We conclude that microtubule growth is directed at dendrite branch points, and that kinesin-2, APC and EB1 are likely to play a role in this process. We propose that is recruited to growing microtubules by +TIPS, and that the motor protein steers growing microtubules at branch points. This represents a newly discovered mechanism to maintain polarized arrays of microtubules.
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DOI：
10.1002/neu.10281
发表时间：
2004-01-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Baas, PW;Buster, DW
通讯作者：
Buster, DW
影响因子：
21.3
作者：
Satoh, Daisuke;Sato, Daichi;Uemura, Tadashi
通讯作者：
Uemura, Tadashi
影响因子：
5.3
作者：
Hu, Xindao;Viesselmann, Chris;Dent, Erik W.
通讯作者：
Dent, Erik W.
影响因子：
3.6
作者：
通讯作者：
--
影响因子：
64.5
作者：
Honnappa, Srinivas;Gouveia, Susana Montenegro;Steinmetz, Michel O.
通讯作者：
Steinmetz, Michel O.