Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study.
Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study.
复制标题
人类免疫缺陷病毒的 gag 和 pol 基因在重组牛痘病毒表达的逆转录病毒样颗粒的形态发生和成熟中的作用：一项超微结构研究。
DOI：
10.1099/0022-1317-72-10-2509
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
T. Kurata
中科院分区：
文献类型：
--
作者：
N. Hoshikawa;A. Kojima;A. Yasuda;E. Takayashiki;S. Masuko;J. Chiba;T. Sata;T. Kurata
用表达人类免疫缺陷病毒（HIV）基因的重组痘苗病毒（RVVs）感染兔上皮细胞RK-13细胞和CD4+人T淋巴细胞系进行了一项超结构研究：单独表达成熟的p17或p24 gag结构域、完整或截断的gag基因、逆转录酶结构域或带有移码突变的gag-pol基因。被rvv感染的细胞产生gag多蛋白，预测Mr大于48K，显示出出芽并将hiv样颗粒释放到细胞外空间。在表达截断gag基因（p17和p24区域）的细胞中未观察到这些颗粒。当整个gag基因和pol基因的蛋白酶区表达时，在细胞外观察到成熟的hiv样颗粒。相比之下，在含有gag-pol基因和移码突变的RVVs感染的细胞中，既不能检测到可识别的出芽结构，也不能检测到细胞外hiv样颗粒。这些结果表明gag基因，特别是它的3'端，对于HIV颗粒的组装是必需的。此外，pol基因的蛋白酶区似乎是HIV颗粒形态成熟所必需的，但gag蛋白的完全蛋白水解裂解可能会阻止芽的形成。
An ultrastructural study was performed on rabbit epithelial RK-13 cells and CD4+ human T lymphocyte lines infected with various recombinant vaccinia viruses (RVVs) expressing genes of human immunodeficiency virus (HIV): the mature p17 or p24 gag domain alone, the entire or truncated gag gene, the reverse transcriptase domain, or the gag-pol genes with a frameshift mutation. Cells infected with RVVs that produced the gag polyprotein with a predicted Mr of more than 48K showed budding and release of HIV-like particles into the extracellular space. These particles were not observed in cells expressing a truncated gag gene (p17 and p24 regions). Mature HIV-like particles were observed extracellularly when the entire gag gene and the protease region of the pol gene were expressed. In contrast, in cells infected with RVVs that contained the gag-pol gene with a frameshift mutation, neither recognizable budding structures nor extracellular HIV-like particles could be detected. These results suggest that the gag gene, particularly its 3' terminus, is necessary for the assembly of HIV particles. In addition, the protease region of the pol gene seems to be required for morphological maturation of HIV particles, but complete proteolytic cleavage of the gag protein may prevent bud formation.
DOI：
10.1073/pnas.86.15.5781
发表时间：
1989-08-01
影响因子：
11.1
作者：
GOTTLINGER, HG;SODROSKI, JG;HASELTINE, WA
通讯作者：
HASELTINE, WA
影响因子：
56.9
作者：
GONDA, MA;WONGSTAAL, F;GILDEN, RV
通讯作者：
GILDEN, RV
与人类和猿猴艾滋病相关的逆转录病毒的分子比较。
影响因子：
3.3
作者：
Bryant,ML;Yamamoto,J;Luciw,P;Munn,R;Marx,P;Higgins,J;Pedersen,N;Levine,A;Gardner,MB
通讯作者：
Gardner,MB