In vitro characterization of Haemonchus contortus trehalose-6-phosphate phosphatase and its immunomodulatory effects on peripheral blood mononuclear cells (PBMCs).
In vitro characterization of Haemonchus contortus trehalose-6-phosphate phosphatase and its immunomodulatory effects on peripheral blood mononuclear cells (PBMCs).
复制标题
捻转血矛线虫海藻糖-6-磷酸磷酸酶的体外表征及其对外周血单核细胞（PBMC）的免疫调节作用
DOI：
10.1186/s13071-021-05115-4
复制
发表时间：
2021-12-20
影响因子：
3.2
通讯作者：
Yan R
中科院分区：
文献类型：
--
作者：
Wen Z;Xie X;Aleem MT;Aimulajiang K;Chen C;Liang M;Song X;Xu L;Li X;Yan R
背景
Background
Trehalose-6-phosphate phosphatase (TPP6) is a key enzyme in the trehalose biosynthesis pathway. The accumulation of TPP6 inside the body is harmful to the pathogen, but almost nothing is currently known about the function of TPP6 from Haemonchus contortus (CRE-GOB-1).
Methods
The H. contortus CRE-GOB-1 (HcGOB) gene was cloned and recombinant protein of GOB (rHcGOB) was expressed; transcription of the HcGOB gene at different developmental stages of H. contortus was then studied. The spatial expression pattern of the HcGOB gene in adult female and male worms was determined by both quantitative real-time PCR (qPCR) and immunofluorescence. The binding of the rHcGOB protein to goat PBMCs was assessed by immunofluorescence assay. The immunomodulatory impacts of rHcGOB on cell proliferation, nitric oxide generation and cytokine secretion were assessed by co-culture of rHcGOB protein with goat PBMCs.
Results
The HcGOB protein was transcribed in eggs, infective third-stage larvae (iL3s) and adults of H. contortus, with the highest transcript levels found in the egg stage. The transcript levels were significantly elevated in iL3s after manual desheathing. HcGOB was widely distributed in adult worms where it was mainly localized in the gut and gonads. rHcGOB was observed to bind to PBMCs and also to be recognized by sera collected from a goat infected with H. contortus. rHcGOB significantly activated the interleukin-10/transforming growth factor β/signal transducer and activator of transcription 3 (IL-10/TGF-β/STAT3) pathway in PBMCs while suppressing the transcription and expression of IL-4 and IL-17.
Conclusions
These results suggest that the HcGOB gene plays an important role in the development, parasitism and reproduction of H. contortus. The rHcGOB protein affected the immunomodulatory function of PBMCs in the in vitro study, suggesting that this protein would be a promising vaccine target.
Graphical Abstract
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影响因子：
6.9
作者：
Joshi, Rohit;Sahoo, Khirod Kumar;Singla-Pareek, Sneh Lata
通讯作者：
Singla-Pareek, Sneh Lata
影响因子：
3.8
作者：
Kushwaha S;Singh PK;Shahab M;Pathak M;Bhattacharya SM
通讯作者：
Bhattacharya SM
DOI：
10.1126/stke.2932005cm9
发表时间：
2005-07-19
期刊：
Science's STKE : signal transduction knowledge environment
影响因子：
--
作者：
Kelly-Welch, Ann;Hanson, Erica M;Keegan, Achsah D
通讯作者：
Keegan, Achsah D
影响因子：
4.8
作者：
Cross, Megan;Lepage, Romain;Hofmann, Andreas
通讯作者：
Hofmann, Andreas
影响因子：
7.8
作者：
Aleem MT;Shi J;Yu Z;Wen Z;Zhang Y;Liang M;Lakho SA;Haseeb M;Ali H;Hassan MW;Song X;Li X;Xu L;Yan R
通讯作者：
Yan R