Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone.
Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone.
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DOI：
10.3390/vaccines9080934
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发表时间：
2021-08-23
期刊：
影响因子：
7.8
通讯作者：
Yan R
中科院分区：
文献类型：
--
作者：
Aleem MT;Shi J;Yu Z;Wen Z;Zhang Y;Liang M;Lakho SA;Haseeb M;Ali H;Hassan MW;Song X;Li X;Xu L;Yan R
旋毛虫病是由包括旋毛虫在内的旋毛虫属引起的食源性人畜共患疾病。本研究克隆了螺旋体膜相关孕酮受体组分-2 （Ts-MAPRC2）基因，并通过蛋白测序分析对其进行了鉴定。对其进行表达、纯化、免疫印迹、与孕酮抗体结合能力、免疫荧光等检测。孕酮（P4）和米非非酮（RU486）对Ts-MAPRC2基因的直接影响是通过体外细胞培养来确定的，在各个发育阶段（肌肉幼虫（ML），雌性成虫（F-AL），雄性成虫（M-AL）和新生幼虫（NBL））中表达水平不同。随后，我们检测了P4、RU486和rt - maprc2 - ab对F-AL和ML期的体外表型影响。随后，我们研究了米非司酮在F-AL期的体内表型效应和相对mRNA表达。我们的研究结果表明，Ts-MAPRC2基因对维持螺旋体雌虫（F-AL）的妊娠至关重要。300 ng/mL P4和100 ng/mL RU486可下调F-AL中Ts-MAPRC2基因（p≤0.05）。这在流产中起重要作用，并可能减少螺旋体在宿主体内的虫负荷。只有30 ng/mL P4显著上调F-AL （p≤0.05）。本研究为重组蛋白（rt - maprc2）抗激素（P4和RU486）药物设计和疫苗治疗以及它们的联合作用控制螺旋体感染提供了新的见解。
Trichinellosis is a foodborne zoonotic disease caused by Trichinella spp., including Trichinella spiralis. In the present study, T. spiralis membrane-associated progesterone receptor component-2 (Ts-MAPRC2) gene was cloned and characterized using protein sequencing analysis. Furthermore, the expression, purification, immunoblot assay, binding ability with progesterone antibody, and immunofluorescence assay were performed. A direct effect of progesterone (P4) and mifepristone (RU486) on the Ts-MAPRC2 gene was determined using in vitro cell culture that showed different expression levels at all developmental stages (muscle larvae (ML), female adult worm (F-AL), male adult worm (M-AL), and newborn larvae (NBL)). Subsequently, the in vitro phenotypic effects of P4, RU486, and rTs-MAPRC2-Ab on F-AL and ML stages were measured. Later, the in vivo phenotypic effect and relative mRNA expression of mifepristone on the F-AL stage were studied. Our results revealed that the Ts-MAPRC2 gene is critical to maintaining pregnancy in the female adult worm (F-AL) of T. spiralis. The 300 ng/mL of P4 and 100 ng/mL of RU486 showed downregulation of the Ts-MAPRC2 gene in F-AL (p ≤ 0.05). This plays an important role in abortion and possibly decreases the worm burden of T. spiralis in the host. Only 30 ng/mL P4 showed significant upregulation in F-AL (p ≤ 0.05). The current study provides new insights regarding the antihormone (P4 and RU486) drug design and vaccine therapy of recombinant (rTs-MAPRC2) protein as well as their combined effects to control T. spiralis infection.
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影响因子：
11.8
作者：
Murrell KD;Pozio E
通讯作者：
Pozio E
影响因子：
3.3
作者：
Piccinni, MP;Scaletti, C;Romagnani, S
通讯作者：
Romagnani, S
影响因子：
2.4
作者：
Nuñez, GG;Gentile, T;Venturiello, SM
通讯作者：
Venturiello, SM
影响因子：
0.9
作者：
Anzaldúa, SR;Camacho-Arroyo, I;Cerbón, MA
通讯作者：
Cerbón, MA
DOI：
10.1007/s12639-017-0891-9
发表时间：
2017-09-01
期刊：
Journal of parasitic diseases : official organ of the Indian Society for Parasitology
影响因子：
--
作者：
Hlaka, Lerato;Chitanga, Simbarashe;Mukaratirwa, Samson
通讯作者：
Mukaratirwa, Samson