ORA47 is a transcriptional regulator of a general stress response hub
ORA47 is a transcriptional regulator of a general stress response hub
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ORA47是一般应激反应中枢的转录调节因子
DOI：
10.1111/tpj.15688
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Dehesh, Katayoon
中科院分区：
文献类型：
--
作者：
Zeng, Liping;Chen, Hao;Wang, Yaqi;Hicks, Derrick;Ke, Haiyan;Pruneda‐Paz, Jose;Dehesh, Katayoon
一般应激反应（GSR）的转录调节因子重新编程选定基因的表达，将信息信号转换为细胞事件，最终表现为植物应对环境挑战的能力。鉴定核心GSR调节蛋白将揭示建立适应性反应的主要模块及其作用模式。为了确定GSR调控组分，我们采用酵母单杂交试验来鉴定与先前建立的功能性GSR基序结合的蛋白质，称为快速应激反应元件（RSRE）。这导致了十八烷响应性AP2/ERF结构域转录因子47（ORA47）的分离，这是一种茉莉酸甲酯诱导的蛋白。随后，使用在渗入4xRSRE：：Luc背景的ORA47功能丧失和获得系中进行的RSRE驱动的荧光素酶（LUC）活性测定来确认ORA47转录活性。此外，遗传学研究证实了钙调蛋白结合转录激活因子3（CAMTA 3）蛋白在诱导RSRE中的主要作用。此外，外源茉莉酸甲酯的应用导致的ORA47和CAMTA 3的转录水平的提高，以及RSRE：：LUC活性的诱导。代谢分析表明，在增加JA水平的相互作用的功能输入的ORA47和CAMTA 3。最后，瞬时测定将JASMONATE ZIM-结构域1（JAZ1）鉴定为RSRE：：LUC活性的阻遏物。总的来说，本研究提供了新的见解的机制，将信息信号转换为适应性反应的初始功能。该机制涉及JA生物合成/信号级联和增强GSR的转录重编程之间的功能相互作用。此外，这些研究结果提供了一个窗口，在建立适应性反应的细胞器内通信的作用。
Transcriptional regulators of the general stress response (GSR) reprogram the expression of selected genes to transduce informational signals into cellular events, ultimately manifested in a plant's ability to cope with environmental challenges. Identification of the core GSR regulatory proteins will uncover the principal modules and their mode of action in the establishment of adaptive responses. To define the GSR regulatory components, we employed a yeast‐one‐hybrid assay to identify the protein(s) binding to the previously established functional GSR motif, termed the rapid stress response element (RSRE). This led to the isolation of octadecanoid‐responsive AP2/ERF‐domain transcription factor 47 (ORA47), a methyl jasmonate inducible protein. Subsequently, ORA47 transcriptional activity was confirmed using the RSRE‐driven luciferase (LUC) activity assay performed in the ORA47 loss‐ and gain‐of‐function lines introgressed into the 4xRSRE::Luc background. In addition, the prime contribution of CALMODULIN‐BINDING TRANSCRIPTIONAL ACTIVATOR3 (CAMTA3) protein in the induction of RSRE was reaffirmed by genetic studies. Moreover, exogenous application of methyl jasmonate led to enhanced levels ofORA47andCAMTA3transcripts, as well as the induction of RSRE::LUC activity. Metabolic analyses illustrated the reciprocal functional inputs of ORA47 and CAMTA3 in increasing JA levels. Lastly, transient assays identified JASMONATE ZIM‐domain1 (JAZ1) as a repressor of RSRE::LUC activity. Collectively, the present study provides fresh insight into the initial features of the mechanism that transduces informational signals into adaptive responses. This mechanism involves the functional interplay between the JA biosynthesis/signaling cascade and the transcriptional reprogramming that potentiates GSR. Furthermore, these findings offer a window into the role of intraorganellar communication in the establishment of adaptive responses.
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影响因子：
20.5
作者：
Pruneda-Paz, Jose L.;Kay, Steve A.
通讯作者：
Kay, Steve A.
DOI：
10.1073/pnas.1602582113
发表时间：
2016-08-02
影响因子：
11.1
作者：
Benn, Geoffrey;Bjornson, Marta;Dehesh, Katayoon
通讯作者：
Dehesh, Katayoon
影响因子：
7.2
作者：
Koo, Abraham J. K.;Gao, Xiaoli;Howe, Gregg A.
通讯作者：
Howe, Gregg A.
影响因子：
7.2
作者：
Laudert, D;Weiler, EW
通讯作者：
Weiler, EW
影响因子：
3.3
作者：
Gasch, AP;Spellman, PT;Brown, PO
通讯作者：
Brown, PO