Rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen
Rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen
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通过早期核抗原单克隆抗体间接免疫过氧化物酶染色快速检测细胞培养物中的巨细胞病毒
DOI：
10.1128/jcm.21.5.669-673.1985
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发表时间：
1985
影响因子：
9.4
通讯作者：
M. Kaplan
中科院分区：
文献类型：
--
作者：
P. Swenson;M. Kaplan
建立了一种快速检测MRC-5细胞中巨细胞病毒（CMV）的方法。将市售的CMV早期核抗原的单克隆抗体用于直接在标准管（16 × 125 mm）中的丙酮固定细胞单层上进行的间接免疫过氧化物酶（IPA）染色程序。在190份临床标本中，30份标本在接种后14天内在组织培养中产生CMV细胞病变效应（TC-CPE），其中28份在48 h后通过IPA染色程序对CMV呈阳性（灵敏度为93%）。160例TC-CPE阴性的临床标本中，IPA染色阳性7例（特异性96%）。然而，这7份标本中有3份在最初的14天孵育期后传代培养时TC-CPE呈阳性，1份标本在CMV致细胞病变效应出现之前被单纯疱疹病毒2型过度生长。30例TC-CPE阳性标本在14天内出现细胞病变的平均时间为6.7天。这些发现表明，IPA染色是快速检测接种临床标本的细胞单层中CMV的有用方法。
A method for the rapid detection of cytomegalovirus (CMV) in MRC-5 cells 48 h after inoculation with clinical specimens was developed. A commercially available monoclonal antibody to a CMV early nuclear antigen was used in an indirect immunoperoxidase (IPA) staining procedure performed directly on acetone-fixed cell monolayers in standard tubes (16 by 125 mm). Of 190 clinical specimens tested, 30 specimens produced CMV cytopathic effect in tissue culture (TC-CPE) within 14 days after inoculation and, of these 30, 28 were positive for CMV after 48 h by the IPA staining procedure (sensitivity, 93%). Of the remaining 160 clinical specimens negative by TC-CPE within 14 days, 7 were positive by the IPA stain (specificity, 96%). However, three of these seven specimens were positive by TC-CPE upon subculture after the initial 14-day incubation period, and one specimen was overgrown by herpes simplex virus type 2 before CMV cytopathic effect could develop. The mean time to appearance of cytopathic effect for the 30 specimens positive by TC-CPE within 14 days was 6.7 days. These findings indicate that this IPA staining is a useful method for the rapid detection of CMV in cell monolayers inoculated with clinical specimens.
通过 DNA 杂交快速检测和定量尿液中的人巨细胞病毒。
DOI：
10.1056/nejm198304213081603
发表时间：
1983
期刊：
The New England journal of medicine
影响因子：
--
作者：
Chou,S;Merigan,TC
通讯作者：
Merigan,TC
DOI：
10.1093/infdis/145.2.647
发表时间：
1982-05
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
P. Griffiths;S. Stagno;R. F. Pass;Richard Smith;C. Alford
通讯作者：
P. Griffiths;S. Stagno;R. F. Pass;Richard Smith;C. Alford
通过开肺活检的接触印迹快速细胞学诊断巨细胞病毒间质性肺炎。
影响因子：
3.5
作者：
Shulman,HM;Hackman,RC;Sale,GE;Meyers,JD
通讯作者：
Meyers,JD