Regeneration of Clivia miniata and assessment of clonal fidelity of plantlets
Regeneration of Clivia miniata and assessment of clonal fidelity of plantlets
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君子兰的再生和植株克隆保真度的评估
DOI：
10.1007/s11240-011-0085-6
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Li Wang
中科院分区：
文献类型：
--
作者：
Xin Sui;Meng Wang;Yue-Jun Hui;Li Wang
以君子兰幼嫩花瓣基部组织和幼嫩子房为外植体，建立了君子兰离体快繁体系。为了诱导愈伤组织，外植体在含有2.22 μM 6-苄基腺嘌呤（BA）和4.52 μM 2，4-二氯苯氧基乙酸（2，4-D）或4.44 μM BA、5.37 μM α-萘乙酸（NAA）和9.05 μM 2，4-D的Murashige和Skoog（MS）培养基上孵育。幼子房在附加8.88 μM BA、10.74 μM NAA和9.05或18.10 μM 2，4-D的MS培养基上诱导出愈伤组织。将愈伤组织转移到附加激动素和NAA的MS培养基上诱导芽器官发生。以花瓣愈伤组织为外植体，在含有2.69 μM NAA和9.29或13.94 μM KT的培养基上，再生频率最高。子房愈伤组织在附加9.29 μM KT和10.74 μM NAA的培养基上分化不定芽的频率最高。总体而言，不同外植体类型表现出不同的器官发生能力，其中，年轻的花瓣有较高的芽再生频率比年轻的子房。将不定芽转移到不含植物生长调节剂的半浓度MS培养基上，生根率最高（98.25 ± 3.04%）。再生植株移栽到混合土中驯化，成活率达96.80%。只有0.6%的再生植株出现形态变化。用根尖染色体计数法确定再生植株为二倍体（2n = 22）。此外，简单重复序列间被用来评估再生植株的遗传保真度。再生植株与母本的遗传相似性为90.5-100.0%，彼此间的遗传相似性为89.0-100.0%。
An efficient in vitro micropropagation system for Clivia miniata Regel was developed using basal tissues of young petals and young ovaries as explants. For callus induction, explants were incubated on Murashige and Skoog (MS) medium containing either 2.22 μM 6-benzyladenine (BA) and 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 4.44 μM BA, 5.37 μM α-naphthaleneacetic acid (NAA), and 9.05 μM 2,4-D. Moreover, callus was induced from young ovaries when these were incubated on MS medium containing 8.88 μM BA, 10.74 μM NAA, and 9.05 or 18.10 μM 2,4-D. Subsequently, callus was transferred to MS medium supplemented with kinetin (KT) and NAA for shoot organogenesis. Frequency of shoot regeneration from petal-derived callus was highest when callus was transferred to medium containing 2.69 μM NAA with either 9.29 or 13.94 μM KT. Shoot regeneration frequency from ovary-derived callus was highest when this callus was transferred to medium containing 9.29 μM KT and 10.74 μM NAA. Overall, different explant types exhibited different organogenic capacities wherein, young petals had higher shoot regeneration frequencies than young ovaries. The highest rooting frequency (98.25 ± 3.04%) was obtained when shoots were transferred to half-strength MS medium without plant growth regulators. Regenerated plantlets were transplanted to soil mix and acclimatized, yielding a 96.80% survival frequency. Only 0.6% of regenerated plantlets exhibited morphological changes. The diploid status (2n = 22) of regenerated plantlets was determined using chromosome counts of root-tips. Moreover, inter-simple sequence repeats were used to assess the genetic fidelity of regenerated plantlets. Overall, regenerated plants shared 90.5–100.0% genetic similarities with mother plants and 89.0–100.0% similarities with each other.
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影响因子：
3
作者：
Guo, DP;Zhu, ZJ;Zheng, SJ
通讯作者：
Zheng, SJ
影响因子：
2.7
作者：
Vikash Sewram1,*;Mark Raynor1, 2;Dulcie Mulholland1;Deshandra Raidoo3
通讯作者：
Vikash Sewram1,*;Mark Raynor1, 2;Dulcie Mulholland1;Deshandra Raidoo3
利用 RAPD、ISSR 和 AFLP 标记绘制菊花 (Chrysanthemum morifolium) 品种的初步遗传连锁图谱
影响因子：
4.3
作者：
Zhang, Fei;Chen, Sumei;Li, Fengtong
通讯作者：
Li, Fengtong
影响因子：
3
作者：
Flores, Patricia Silva;Otoni, Wagner Campos;Bruckner, Claudio Horst
通讯作者：
Bruckner, Claudio Horst
DOI：
10.1111/j.1365-313x.2003.02014.x
发表时间：
2004-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Fei Yu;Sungsoon Park;S. Rodermel
通讯作者：
Fei Yu;Sungsoon Park;S. Rodermel