Genomewide identification of PPR gene family and prediction analysis on restorer gene in Gossypium
Genomewide identification of PPR gene family and prediction analysis on restorer gene in Gossypium
复制标题
棉花PPR基因家族全基因组鉴定及恢复基因预测分析
DOI：
10.1007/s12041-018-0993-x
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发表时间：
2018-09
影响因子：
1.5
通讯作者：
Jinping Hua
中科院分区：
文献类型：
--
作者：
Nan Zhao;Yumei Wang;Jinping Hua
五肽重复序列（Pentatricopeptide repeat， PPR）基因家族在植物生长和细胞器基因表达调控中起着重要作用。一些PPR基因与植物的育性恢复有关，但在棉属中没有详细的信息。在本研究中，我们分别在Gossypium raimondii （$$\hbox {D}_{5}$$ D5）和G. arboreum （$$\hbox {A}_{2}$$ A2）基因组中鉴定了482个和433个PPR同源物。大多数小反刍兽疫同源物在整个染色体上均匀分布。通过对G. raimondii （$$\hbox {D}_{5}$$ D5）、G. arboreum （$$\hbox {A}_{2}$$ A2）和G. hirsutum基因组中PPR基因的进化分析，发现8个PPR基因与其他物种的恢复基因聚集在一起。大部分棉花PPR基因不含内含子，$$\upalpha $$ α-螺旋比例高，具有典型的PPR蛋白三级结构。基于生物信息学分析，在线粒体中定位了8个PPR基因，编码具有蛋白质结合活性和细胞器RNA代谢功能的典型P亚家族蛋白。通过RNA-seq和qRT-PCR进一步验证，两个PPR候选基因Gorai.005G0470 （$$\hbox {D}_{5}$$ D5）和Cotton＿A＿08373 （$$\hbox {A}_{2}$$ A2）在可育系中表达量明显高于不育系。这些结果为棉属植物小反刍兽疫基因的进化提供了新的见解。
Pentatricopeptide repeat (PPR) gene family plays an essential role in the regulation of plant growth and organelle gene expression. Some PPR genes are related to fertility restoration in plant, but there is no detailed information in Gossypium. In the present study, we identified 482 and 433 PPR homologues in Gossypium raimondii ($$\hbox {D}_{5}$$D5) and G. arboreum ($$\hbox {A}_{2}$$A2) genomes, respectively. Most PPR homologues showed an even distribution on the whole chromosomes. Given an evolutionary analysis to PPR genes from G. raimondii ($$\hbox {D}_{5}$$D5), G. arboreum ($$\hbox {A}_{2}$$A2) and G. hirsutum genomes, eight PPR genes were clustered together with restoring genes of other species. Most cotton PPR genes were qualified with no intron, high proportion of $$\upalpha $$α-helix and classical tertiary structure of PPR protein. Based on bioinformatics analyses, eight PPR genes were targeted in mitochondrion, encoding typical P subfamily protein with protein binding activity and organelle RNA metabolism in function. Further verified by RNA-seq and quantitative real-time PCR (qRT-PCR) analyses, two PPR candidate genes, Gorai.005G0470 ($$\hbox {D}_{5}$$D5) and Cotton_A_08373 ($$\hbox {A}_{2}$$A2), were upregulated in fertile line than sterile line. These results reveal new insights into PPR gene evolution in Gossypium.
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影响因子：
14.9
作者：
Mistry J;Finn RD;Eddy SR;Bateman A;Punta M
通讯作者：
Punta M
影响因子：
4.6
作者：
Melonek J;Stone JD;Small I
通讯作者：
Small I
DOI：
10.2134/agronmonogr57.2013.0020
发表时间：
2015-10
期刊：
--
影响因子：
--
作者：
J. Wendel;C. Grover
通讯作者：
J. Wendel;C. Grover
有害的线粒体-核相互作用导致水稻细胞质雄性不育
影响因子：
30.8
作者：
Luo, Dangping;Xu, Hong;Liu, Yao-Guang
通讯作者：
Liu, Yao-Guang
影响因子：
3.1
作者：
Xia, X;Xie, Z
通讯作者：
Xie, Z