Genome Sequencing and Analysis of Trichoderma (Hypocreaceae) Isolates Exhibiting Antagonistic Activity against the Papaya Dieback Pathogen, Erwinia mallotivora.
Genome Sequencing and Analysis of Trichoderma (Hypocreaceae) Isolates Exhibiting Antagonistic Activity against the Papaya Dieback Pathogen, Erwinia mallotivora.
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DOI：
10.3390/jof8030246
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发表时间：
2022-02-28
期刊：
影响因子：
--
通讯作者：
Alberti F
中科院分区：
文献类型：
--
作者：
Tamizi AA;Mat-Amin N;Weaver JA;Olumakaiye RT;Akbar MA;Jin S;Bunawan H;Alberti F
mallotivora Erwinia mallotivora是木瓜枯死病的致病因子，是一种毁灭性的病原体，导致马来西亚木瓜出口大幅减少，并影响邻国的木瓜作物。一些研究已经报道了能够抑制mallotivora的细菌种类，但拮抗真菌的可用性仍然未知。在本研究中，发现5株马来西亚根际木霉分离株的菌丝悬浮液在共培养过程中对mallotivora菌表现出显著的拮抗作用。我们进一步鉴定了三株分离菌株，koningiopsis Trichoderma UKM-M-UW RA5， UKM-M-UW RA6和UKM-M-UW RA3a，在平板抑制实验中显示出明显的生长抑制区。通过结合牛津纳米孔和Illumina测序技术对这三种菌株的基因组进行的研究突出了潜在的次级代谢物途径，这些途径可能支持它们的抗菌特性。基于这些发现，真菌分离物被证明是有用的潜在生物防治剂，基因组数据为进一步探索其抗菌活性背后的潜在分子机制提供了可能，并具有潜在的合成生物学应用。
Erwinia mallotivora, the causal agent of papaya dieback disease, is a devastating pathogen that has caused a tremendous decrease in Malaysian papaya export and affected papaya crops in neighbouring countries. A few studies on bacterial species capable of suppressing E. mallotivora have been reported, but the availability of antagonistic fungi remains unknown. In this study, mycelial suspensions from five rhizospheric Trichoderma isolates of Malaysian origin were found to exhibit notable antagonisms against E. mallotivora during co-cultivation. We further characterised three isolates, Trichoderma koningiopsis UKM-M-UW RA5, UKM-M-UW RA6, and UKM-M-UW RA3a, that showed significant growth inhibition zones on plate-based inhibition assays. A study of the genomes of the three strains through a combination of Oxford nanopore and Illumina sequencing technologies highlighted potential secondary metabolite pathways that might underpin their antimicrobial properties. Based on these findings, the fungal isolates are proven to be useful as potential biological control agents against E. mallotivora, and the genomic data opens possibilities to further explore the underlying molecular mechanisms behind their antimicrobial activity, with potential synthetic biology applications.
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DOI：
10.1631/jzus.2005.b0100
发表时间：
2005-02-01
期刊：
Journal of Zhejiang University. Science. B
影响因子：
--
作者：
Druzhinina, Irina;Kubicek, Christian P
通讯作者：
Kubicek, Christian P
影响因子：
5
作者：
Alberti, Fabrizio;Foster, Gary D.;Bailey, Andy M.
通讯作者：
Bailey, Andy M.
影响因子：
--
作者：
Juri, Nor Mustaiqazah;Samsuddin, Aimera Farhana;Abu Bakar, Norliza
通讯作者：
Abu Bakar, Norliza
影响因子：
6.7
作者：
Gil, S. Vargas;Pastor, S.;March, G. J.
通讯作者：
March, G. J.
影响因子：
5.1
作者：
Berkaew, Pitchapa;Soonthornchareonnon, Noppamas;Isaka, Masahiko
通讯作者：
Isaka, Masahiko