Localization of a GABA transporter to glial cells in the developing and adult olfactory pathway of the moth Manduca sexta.
Localization of a GABA transporter to glial cells in the developing and adult olfactory pathway of the moth Manduca sexta.
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DOI：
10.1002/cne.22244
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发表时间：
2010-03-15
期刊：
影响因子：
--
通讯作者：
Tolbert LP
中科院分区：
文献类型：
--
作者：
Oland LA;Gibson NJ;Tolbert LP
神经胶质细胞在蛾的发育和成虫嗅叶（触角）中起着重要的作用。在发育早期，神经胶质细胞占据发育中的嗅觉通路的离散区域，gaba能神经元的过程延伸到其中的一些区域。由于已知GABA在多种系统中具有发育作用，我们探索了神经胶质细胞表达GABA转运体的可能性，该转运体可以调节嗅觉神经元和神经胶质细胞暴露的GABA水平。利用一种针对高亲和性、与已知哺乳动物GABA转运蛋白高度同源的m.s sexta GABA转运蛋白的抗体，我们发现GABA转运蛋白在蜕变成体发育过程中定位于中枢来源的神经胶质细胞亚群。这种转运体在一部分神经药物相关的神经胶质细胞中持续存在到成年期，但通过光镜和电子显微镜水平的免疫细胞化学测定其分布模式表明，它不能调节突触间隙中GABA的浓度。相反，它的作用更可能是调节肾小球神经细胞内的细胞外GABA水平。嗅觉受体轴突生长后，分选区胶质细胞的表达消失，但如果GABA调节轴突生长，分选区胶质细胞的表达可能在轴突生长过程中起重要作用。神经胶质细胞吸收GABA，而这种吸收可以被DABA阻断。这是第一个分子证据表明中枢神经胶质细胞群在这一途径是异质的。
Glial cells have several critical roles in the developing and adult olfactory (antennal) lobe of the moth Manduca sexta. Early in development, glial cells occupy discrete regions of the developing olfactory pathway and processes of GABAergic neurons extend into some of these regions. Because GABA is known to have developmental effects in a variety of systems, we explored the possibility that the glial cells express a GABA transporter that could regulate GABA levels to which olfactory neurons and glial cells are exposed. Using an antibody raised against a characterized high-affinity M. sexta GABA transporter with high sequence homology to known mammalian GABA transporters, we found that the GABA transporter is localized to subsets of centrally derived glial cells during metamorphic adult development. The transporter persists into adulthood in a subset of the neuropil-associated glial cells, but its distribution pattern as determined by light- and electron-microscopic-level immunocytochemistry indicates that it could not serve to regulate GABA concentration in the synaptic cleft. Rather its role is more likely to regulate extracellular GABA levels within the glomerular neuropil. Expression in the sorting zone glial cells disappears after the period of olfactory receptor axon ingrowth, but may be important during ingrowth if GABA regulates axon growth. Glial cells take up GABA, and that uptake can be blocked by DABA. This is the first molecular evidence that the central glial cell population in this pathway is heterogeneous.
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影响因子：
3.8
作者：
Gao, XJ;McLean, H;Donly, C
通讯作者：
Donly, C
影响因子：
2.5
作者：
BARBIN, G;POLLARD, H;BENARI, Y
通讯作者：
BENARI, Y
影响因子：
56.9
作者：
GUASTELLA, J;NELSON, N;KANNER, BI
通讯作者：
KANNER, BI
DOI：
10.1073/pnas.76.1.499
发表时间：
1979-01-01
影响因子：
11.1
作者：
HILDEBRAND, JG;HALL, LM;OSMOND, BC
通讯作者：
OSMOND, BC
影响因子：
16.2
作者：
DiGregorio, DA;Nusser, Z;Silver, RA
通讯作者：
Silver, RA