Novel hepatoviruses in synanthropic bats in the upper Midwestern United States.

Novel hepatoviruses in synanthropic bats in the upper Midwestern United States.
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DOI:
10.1007/s00705-022-05610-8
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发表时间:
2022-12
影响因子:
2.7
通讯作者:
--
中科院分区:
医学4区
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从南达科他州因人类接触而送检狂犬病病毒检测的一只棕蝠(Eptesicus fuscus)中分离出了庚型肝病毒(hepatovirus G)近乎完整的基因组序列。预测的多聚蛋白序列与从加纳蝙蝠中发现的G1和G2基因型分别有78.2%和74.4%的同一性。对90只棕蝠进行定量聚合酶链反应(PCR)显示,8只(8.9%)庚型肝病毒检测呈阳性。对5个阳性样本的基因组VP2区域进行靶向测序,结果显示与庚型肝病毒Ef15893株的同一性>99%,这表明庚型肝病毒在中西部上游地区的棕蝠中普遍传播。 网络版包含补充材料,可在10.1007/s00705 - 2022 - 05610 - 8获取。
A nearly complete genome sequence of hepatovirus G was isolated from an Eptesicus fuscus bat submitted for rabies virus testing due to human exposure in South Dakota. The predicted polyprotein sequence was 78.2% and 74.4% identical to genotypes G1 and G2, respectively, recovered from bats in Ghana. Quantitative PCR on 90 E. fuscus bats showed that eight (8.9%) were positive for hepatovirus G. Targeted sequencing of the VP2 region of the genome for five positive samples showed >99% identity to hepatovirus G strain Ef15893, demonstrating that hepatovirus G commonly circulates in E. fuscus bats in the upper Midwest. The online version contains supplementary material available at 10.1007/s00705-022-05610-8.
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