Comparative Aerial and Ground Based High Throughput Phenotyping for the Genetic Dissection of NDVI as a Proxy for Drought Adaptive Traits in Durum Wheat.
Comparative Aerial and Ground Based High Throughput Phenotyping for the Genetic Dissection of NDVI as a Proxy for Drought Adaptive Traits in Durum Wheat.
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DOI：
10.3389/fpls.2018.00893
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发表时间：
2018
影响因子：
5.6
通讯作者：
Tuberosa R
中科院分区：
文献类型：
--
作者：
Condorelli GE;Maccaferri M;Newcomb M;Andrade-Sanchez P;White JW;French AN;Sciara G;Ward R;Tuberosa R
高通量表型分析平台(HTPP)为更有效地剖析干旱适应性状的遗传基础提供了新的机会。这项全基因组关联研究比较了两个无人机(UAVs)和一个用于测量归一化差异植被指数(NDVI)的地面平台对248个硬粒小麦(Triticum turgiumL.SSP)的结果。硬粒土(Durum Desf.)不同生育期和水分条件下的供试材料。我们的结果表明，机载平台检测NDVI数量性状基因座(QTL)的能力增强，特别是在终端干旱胁迫下，分别检测到22个和16个单QTL，基于多QTL模型的表型变异分别可解释89.6%和64.7%。此外，还研究了在末期干旱胁迫下硬骨板的叶片叶绿素含量(SPAD)、卷叶和干生物量。共有46个显著QTL影响跨平台NDVI，其中22个QTL对叶片绿度、2个对卷叶、10个对生物量表现伴生效应。在位于第1A、1B、2B、4B、5B、6B和7B染色体上的9个QTL热点中，有8个QTL本身表现出与物候无关的效应。
High-throughput phenotyping platforms (HTPPs) provide novel opportunities to more effectively dissect the genetic basis of drought-adaptive traits. This genome-wide association study (GWAS) compares the results obtained with two Unmanned Aerial Vehicles (UAVs) and a ground-based platform used to measure Normalized Difference Vegetation Index (NDVI) in a panel of 248 elite durum wheat (Triticum turgidum L. ssp. durum Desf.) accessions at different growth stages and water regimes. Our results suggest increased ability of aerial over ground-based platforms to detect quantitative trait loci (QTL) for NDVI, particularly under terminal drought stress, with 22 and 16 single QTLs detected, respectively, and accounting for 89.6 vs. 64.7% phenotypic variance based on multiple QTL models. Additionally, the durum panel was investigated for leaf chlorophyll content (SPAD), leaf rolling and dry biomass under terminal drought stress. In total, 46 significant QTLs affected NDVI across platforms, 22 of which showed concomitant effects on leaf greenness, 2 on leaf rolling and 10 on biomass. Among 9 QTL hotspots on chromosomes 1A, 1B, 2B, 4B, 5B, 6B, and 7B that influenced NDVI and other drought-adaptive traits, 8 showed per se effects unrelated to phenology.
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影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
5.4
作者：
Letta, Tesfaye;Maccaferri, Marco;Tuberosa, Roberto
通讯作者：
Tuberosa, Roberto
影响因子：
56.9
作者：
Avni, Raz;Nave, Moran;Distelfeld, Assaf
通讯作者：
Distelfeld, Assaf
DOI：
10.1073/pnas.1421533112
发表时间：
2015-03-17
影响因子：
11.1
作者：
Kelley, Colin P.;Mohtadi, Shahrzad;Kushnir, Yochanan
通讯作者：
Kushnir, Yochanan
影响因子：
3.4
作者：
Bort, J;Casadesus, J;Araus, JL
通讯作者：
Araus, JL