Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, Calloselasma rhodostoma (Malayan Pit Viper) and Ophiophagus hannah (King Cobra).
Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, Calloselasma rhodostoma (Malayan Pit Viper) and Ophiophagus hannah (King Cobra).
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DOI：
10.3390/toxins10110434
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发表时间：
2018-10-26
期刊：
影响因子：
4.2
通讯作者：
Hodgson WC
中科院分区：
文献类型：
--
作者：
Kunalan S;Othman I;Syed Hassan S;Hodgson WC
Rhodostoma Calloselasma（CR）和Ophiophagus hannah（OH）是在马来西亚发现的两种医学上重要的蛇。虽然一些研究已经描述了这些毒液的生物学特性，但摄食和环境条件也会影响蛇毒毒素的浓度和分布，导致毒液成分的变化。因此，结合蛋白质组学的方法，使用鸟枪和凝胶过滤色谱，串联质谱分析，被用来检查这些马来西亚蛇毒液的组成。结果表明，在马来西亚红口丽金龟和眼镜王蛇中分别发现了114个蛋白（15个毒素家族）和176个蛋白（20个毒素家族）。黄素单胺氧化酶，磷脂酶A2，磷酸二酯酶，蛇毒金属蛋白酶，丝氨酸蛋白酶毒素家族被确定在这两种毒液。在CR毒液中首次鉴定出氨肽酶、氨酰肽环转移酶沿着锚蛋白重复序列，在OH毒液中首次鉴定出胰岛素、C型凝集素/snaclecs、肝细胞生长因子、巨噬细胞集落刺激因子和肿瘤坏死因子。我们结合蛋白质组学的方法已经确定了一个全面的阿森纳的毒素在CR和OH毒液。这些数据可用于改进抗蛇毒血清的生产，了解envenoming的病理影响，并发现具有医学和/或生物技术价值的生物活性肽。
Calloselasma rhodostoma (CR) and Ophiophagus hannah (OH) are two medically important snakes found in Malaysia. While some studies have described the biological properties of these venoms, feeding and environmental conditions also influence the concentration and distribution of snake venom toxins, resulting in variations in venom composition. Therefore, a combined proteomic approach using shotgun and gel filtration chromatography, analyzed by tandem mass spectrometry, was used to examine the composition of venoms from these Malaysian snakes. The analysis revealed 114 proteins (15 toxin families) and 176 proteins (20 toxin families) in Malaysian Calloselasma rhodostoma and Ophiophagus hannah species, respectively. Flavin monoamine oxidase, phospholipase A2, phosphodiesterase, snake venom metalloproteinase, and serine protease toxin families were identified in both venoms. Aminopeptidase, glutaminyl-peptide cyclotransferase along with ankyrin repeats were identified for the first time in CR venom, and insulin, c-type lectins/snaclecs, hepatocyte growth factor, and macrophage colony-stimulating factor together with tumor necrosis factor were identified in OH venom for the first time. Our combined proteomic approach has identified a comprehensive arsenal of toxins in CR and OH venoms. These data may be utilized for improved antivenom production, understanding pathological effects of envenoming, and the discovery of biologically active peptides with medical and/or biotechnological value.
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影响因子：
2.8
作者：
Calvete, Juan J.
通讯作者：
Calvete, Juan J.
影响因子：
3.3
作者：
de Oliveira, Isadora Sousa;Cardoso, Iara Aime;Arantes, Eliane Candiani
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作者：
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DOI：
10.1016/j.cbpc.2004.09.020
发表时间：
2005-01-01
影响因子：
2.2
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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