Polymorphism analysis in identification of genetic variation and relationships among Stylosanthes species

Polymorphism analysis in identification of genetic variation and relationships among Stylosanthes species
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多态性分析在柱花植物遗传变异及亲缘关系鉴定中的应用

DOI:
10.1007/s13205-017-0705-x
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发表时间:
2017-04
期刊:
影响因子:
2.8
通讯作者:
白昌军
白昌军
中科院分区:
工程技术4区
文献类型:
--
作者:
黄春琼;刘国道;白昌军

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利用序列相关扩增多态性标记对柱柱花属6种柱柱花(S. guianensis、S. hamata、S. scabra、S. seabrana、S. macrocephala和S. capitata) 148份材料的遗传变异和亲缘关系进行了分析。结果表明,18对引物共产生138个不同的片段。片段大小在150 ~ 2000 bp之间。148份材料的遗传相似系数为0.51 ~ 0.99,平均为0.79。18对引物产生的有效等位基因数(ne)平均为1.3552,范围为1.2069 ~ 1.6080;Nei’s基因多样性(He)范围为0.1304 ~ 0.3207,平均值为0.2070;Shannon’s information index (I)均值为0.3213,取值范围为0.2233 ~ 0.4582。采用算术平均值为0.69的非加权配对组方法将148个条目分成两个不同的组。一个类群属于贵州南方,另一个类群属于非贵州南方。guianensis类型。本研究证实柱花草具有丰富的遗传变异,为柱花草新品种选育提供了良好的基础。本研究表明,SRAP技术是一种可靠的柱花草品种鉴别和亲缘关系鉴定工具。
A total of 148 accessions representing six important species of the genus Stylosanthes, including S. guianensis, S. hamata, S. scabra, S. seabrana, S. macrocephala, and S. capitata, were used to evaluate genetic variation and relationships using sequence-related amplified polymorphism markers. The results showed that the 18 selected primer pairs generated 138 distinct fragments. The fragment sizes ranged from 150 to 2000 bp. Genetic similarity coefficients among the 148 accessions ranged from 0.51 to 0.99, with an average of 0.79. The effective allele number (ne) generated by the 18 primer pairs averaged 1.3552 and ranged from 1.2069 to 1.6080; Nei’s gene diversity (He) ranged from 0.1304 to 0.3207, with an average of 0.2070; and Shannon’s information index (I) averaged 0.3213 and ranged from 0.2233 to 0.4582. The unweighted pair-group method with arithmetic averages at the 0.69 similarity level separated the 148 accessions into two distinct groups. One group belonged to S. guianensis, and the other group belonged to the non-S. guianensis type. This study verified that Stylosanthes have rich genetic variation, which is an excellent basis for Stylosanthes breeding for new cultivars. This study demonstrates that the SRAP technique is a reliable tool for differentiating Stylosanthes accessions and for discerning genetic relationship among them.
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