Polymorphism analysis in identification of genetic variation and relationships among Stylosanthes species
Polymorphism analysis in identification of genetic variation and relationships among Stylosanthes species
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多态性分析在柱花植物遗传变异及亲缘关系鉴定中的应用
DOI:
10.1007/s13205-017-0705-x
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发表时间:
2017-04
期刊:
影响因子:
2.8
通讯作者:
白昌军
中科院分区:
文献类型:
--
作者:
黄春琼;刘国道;白昌军
A total of 148 accessions representing six important species of the genus Stylosanthes, including S. guianensis, S. hamata, S. scabra, S. seabrana, S. macrocephala, and S. capitata, were used to evaluate genetic variation and relationships using sequence-related amplified polymorphism markers. The results showed that the 18 selected primer pairs generated 138 distinct fragments. The fragment sizes ranged from 150 to 2000 bp. Genetic similarity coefficients among the 148 accessions ranged from 0.51 to 0.99, with an average of 0.79. The effective allele number (ne) generated by the 18 primer pairs averaged 1.3552 and ranged from 1.2069 to 1.6080; Nei’s gene diversity (He) ranged from 0.1304 to 0.3207, with an average of 0.2070; and Shannon’s information index (I) averaged 0.3213 and ranged from 0.2233 to 0.4582. The unweighted pair-group method with arithmetic averages at the 0.69 similarity level separated the 148 accessions into two distinct groups. One group belonged to S. guianensis, and the other group belonged to the non-S. guianensis type. This study verified that Stylosanthes have rich genetic variation, which is an excellent basis for Stylosanthes breeding for new cultivars. This study demonstrates that the SRAP technique is a reliable tool for differentiating Stylosanthes accessions and for discerning genetic relationship among them.
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影响因子:
--
作者:
Liu Guodao;C. Phaikaew;W. Stür
通讯作者:
Liu Guodao;C. Phaikaew;W. Stür
影响因子:
2.9
作者:
Santos-Garcia MO;Karia CT;Resende RM;Chiari L;Vieira ML;Zucchi MI;Souza AP
通讯作者:
Souza AP
DOI:
10.1080/03746609408684819
发表时间:
1994
期刊:
Botanical Journal of Scotland
影响因子:
--
作者:
Everley J. Glover;A. Gillies;R. Abbott
通讯作者:
Everley J. Glover;A. Gillies;R. Abbott
影响因子:
5.4
作者:
C. Liu;J. Musial;B. Thomas
通讯作者:
C. Liu;J. Musial;B. Thomas
DOI:
10.3109/10425179909033948
发表时间:
1999
期刊:
DNA sequence : the journal of DNA sequencing and mapping
影响因子:
--
作者:
J. Vander Stappen;G. Volckaert
通讯作者:
J. Vander Stappen;G. Volckaert