Effect of commercially purified deoxynivalenol and zearalenone mycotoxins on microbial diversity of pig cecum contents.
Effect of commercially purified deoxynivalenol and zearalenone mycotoxins on microbial diversity of pig cecum contents.
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商业纯化脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮真菌毒素对猪盲肠内容物微生物多样性的影响。
DOI：
10.5713/ajas.20.0137
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发表时间：
2021-03
影响因子：
2.2
通讯作者：
Lee SD
中科院分区：
文献类型：
--
作者：
Reddy KE;Kim M;Kim KH;Ji SY;Baek Y;Chun JL;Jung HJ;Choe C;Lee HJ;Kim M;Lee SD
脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZEN）是经常污染玉米和谷物的真菌毒素，对人类和动物的健康构成风险，并导致经济损失。肠道微生物组已被证明有助于对抗这些毒素的影响，据报道，某些微生物对解毒过程有显着贡献。我们检查了三个不同饮食组的猪的盲肠内容物（对照，以及用8 mg DON/kg饲料或0.8mg ZEN/kg饲料污染的饮食）。从盲肠内容物中获得细菌16 S rRNA基因扩增子，并通过下一代测序进行评价。总共生成了2，539，288个序列，具有约500个核苷酸读取长度。厚壁菌门、拟杆菌门和变形菌门是优势门，占所有三个类群的96%以上。乳杆菌属、拟杆菌属、巨球形菌属和弯曲杆菌属显示出作为每组的生物标志物的潜力。特别地，乳酸杆菌和拟杆菌在DON和ZEN组中比在对照组中更丰富。此外，在三组中检测到52，414个操作分类单位;拟杆菌属、乳杆菌属、弯曲杆菌属和普雷沃氏菌属的操作分类单位最占优势，并且组间差异显著。因此，饲料被DON和ZEN污染会影响盲肠微生物群，而乳酸杆菌和拟杆菌高度丰富，并对宿主生理产生积极影响。乳酸杆菌和拟杆菌在解毒和提高免疫反应的过程中起着关键作用。因此，我们认为，这些结果可能是有用的，以确定是否在肠道微植物群的干扰，如DON和ZEN的毒性作用，可以通过调节肠道细菌植物群治疗。
Deoxynivalenol (DON) and zearalenone (ZEN) are mycotoxins that frequently contaminate maize and grain cereals, imposing risks to the health of both humans and animals and leading to economic losses. The gut microbiome has been shown to help combat the effects of such toxins, with certain microorganisms reported to contribute significantly to the detoxification process. We examined the cecum contents of three different dietary groups of pigs (control, as well as diets contaminated with 8 mg DON/kg feed or 0.8 mg ZEN/kg feed). Bacterial 16S rRNA gene amplicons were acquired from the cecum contents and evaluated by next-generation sequencing. A total of 2,539,288 sequences were generated with ~500 nucleotide read lengths. Firmicutes, Bacteroidetes, and Proteobacteria were the dominant phyla, occupying more than 96% of all three groups. Lactobacillus, Bacteroides, Megasphaera, and Campylobacter showed potential as biomarkers for each group. Particularly, Lactobacillus and Bacteroides were more abundant in the DON and ZEN groups than in the control. Additionally, 52,414 operational taxonomic units were detected in the three groups; those of Bacteroides, Lactobacillus, Campylobacter, and Prevotella were most dominant and significantly varied between groups. Hence, contamination of feed by DON and ZEN affected the cecum microbiota, while Lactobacillus and Bacteroides were highly abundant and positively influenced the host physiology. Lactobacillus and Bacteroides play key roles in the process of detoxification and improving the immune response. We, therefore, believe that these results may be useful for determining whether disturbances in the intestinal microflora, such as the toxic effects of DON and ZEN, can be treated by modulating the intestinal bacterial flora.
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DOI：
10.5713/ajas.18.0801
发表时间：
2020-02-01
期刊：
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
影响因子：
--
作者：
Ertekin, Ibrahim;Kizilsimsek, Mustafa
通讯作者：
Kizilsimsek, Mustafa
影响因子：
4.4
作者：
El-Nezami, H;Polychronaki, N;Mykkänen, H
通讯作者：
Mykkänen, H
DOI：
10.5713/ajas.19.0135
发表时间：
2019-12-01
期刊：
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
影响因子：
--
作者：
Jia, Tingting;Sun, Zhiqiang;Yu, Zhu
通讯作者：
Yu, Zhu
影响因子：
2
作者：
El-Nezami, H;Mykkänen, H;Ahokas, J
通讯作者：
Ahokas, J
DOI：
10.5713/ajas.18.0941
发表时间：
2020-03-01
期刊：
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
影响因子：
--
作者：
Hu, Ping;Niu, Qingyan;Zhu, Weiyun
通讯作者：
Zhu, Weiyun