Genetic diversity of Mycobacterium tuberculosis Complex in Jos, Nigeria.
Genetic diversity of Mycobacterium tuberculosis Complex in Jos, Nigeria.
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DOI：
10.1186/1471-2334-10-189
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发表时间：
2010-06-26
影响因子：
3.7
通讯作者：
Dahle UR
中科院分区：
文献类型：
--
作者：
Ani A;Bruvik T;Okoh Y;Agaba P;Agbaji O;Idoko J;Dahle UR
尼日利亚的结核病发病率很高，有必要对该国的结核分枝杆菌复合体（MTC）进行基因分型研究，以提高我们对这一流行病的了解。2006-2008年期间，从尼日利亚中北部乔斯地区肺结核病例中分离出结核分枝杆菌分离株。采用Lowenstein Jensen （LJ）斜坡比例法对111株分枝杆菌中的77株进行药敏试验（DST），并用spoligotyping对分枝杆菌DNA进行基因分型。使用SpolDB4数据库和基于模型的程序“spotcluster”将分离物分配到家族、亚家族和变体。从尼日利亚高原州乔斯市连续结核病患者中分离出的111株肺分离株为孢子型。84株（76%）分离株属于拉丁美洲地中海（LAM）科。其中78个分离株属于LAM10谱系。其中66个具有相同的斑点图案。获得的药物敏感性谱与任何斑点型不一致。少数结核分枝杆菌谱系的优势表明，要么传播率高，要么频繁输入密切相关的菌株，要么是高度保守的基因型。相同LAM10的优势是否代表疫情仍有待证实。Spoligotyping有助于全面了解当地结核病流行情况。本研究表明，尼日利亚乔斯的结核病发病率可能是由少数成功的结核分枝杆菌家族引起的，其中以LAM10家族为主。
Nigeria has a high tuberculosis incidence, and genotyping studies of Mycobacterium tuberculosis Complex (MTC) in the country are necessary in order to improve our understanding of the epidemic. Isolates of MTC were isolated from cases of pulmonary tuberculosis in Jos, North Central region of Nigeria during 2006-2008. Drug susceptibility test (DST) was performed on 77 of 111 isolates by proportion method on Lowenstein Jensen (LJ) slope while genotyping of mycobacterial DNA was performed by spoligotyping. The SpolDB4 database and the model-based program 'spotclust' were used to assign isolates to families, subfamilies and variants. A total of 111 pulmonary isolates from consecutive tuberculosis patients in the city of Jos, Plateau State, Nigeria were spoligotyped. A total of 84 (76%) of the isolates belonged to the Latin American Mediterranean (LAM) family. Of these, 78 isolates were assigned to the LAM10 lineage. Among these, 66 exhibited identical spoligopatterns. Drug susceptibility profiles obtained were not consistently associated with any spoligopattern. The dominance of few M. tuberculosis lineages suggests either a high rate of transmission, frequent import of closely related strains, or a highly conserved genotype. It remains to be confirmed whether the predominance of identical LAM10 represent an outbreak. Spoligotyping was useful to gain an overall understanding of the local TB epidemic. This study demonstrated that the incidence of TB in Jos, Nigeria may be caused by a few successful M. tuberculosis families, dominated by the LAM10 family.
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影响因子：
8.4
作者：
Cadmus, S. I. B.;Jenkins, A. O.;Godfroid, J.;Osinusi, K.;Adewole, I. F.;Murphy, R. L.;Taiwo, B. O.
通讯作者：
Taiwo, B. O.
影响因子：
3.7
作者：
Abadía E;Sequera M;Ortega D;Méndez MV;Escalona A;Da Mata O;Izarra E;Rojas Y;Jaspe R;Motiwala AS;Alland D;de Waard J;Takiff HE
通讯作者：
Takiff HE
影响因子：
9.4
作者：
Kamerbeek, J;Schouls, L;vanEmbden, J
通讯作者：
vanEmbden, J
影响因子：
1.9
作者：
Ani, Agatha;Okpe, Silvanis;Idoko, John
通讯作者：
Idoko, John
影响因子：
3.7
作者：
Kinander W;Bruvik T;Dahle UR
通讯作者：
Dahle UR