A Satellite-Free Centromere in Equus przewalskii Chromosome 10.
A Satellite-Free Centromere in Equus przewalskii Chromosome 10.
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DOI：
10.3390/ijms24044134
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发表时间：
2023-02-18
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
In mammals, centromeres are epigenetically specified by the histone H3 variant CENP-A and are typically associated with satellite DNA. We previously described the first example of a natural satellite-free centromere on Equus caballus chromosome 11 (ECA11) and, subsequently, on several chromosomes in other species of the genus Equus. We discovered that these satellite-free neocentromeres arose recently during evolution through centromere repositioning and/or chromosomal fusion, after inactivation of the ancestral centromere, where, in many cases, blocks of satellite sequences were maintained. Here, we investigated by FISH the chromosomal distribution of satellite DNA families in Equus przewalskii (EPR), demonstrating a good degree of conservation of the localization of the major horse satellite families 37cen and 2PI with the domestic horse. Moreover, we demonstrated, by ChIP-seq, that 37cen is the satellite bound by CENP-A and that the centromere of EPR10, the ortholog of ECA11, is devoid of satellite sequences. Our results confirm that these two species are closely related and that the event of centromere repositioning which gave rise to EPR10/ECA11 centromeres occurred in the common ancestor, before the separation of the two horse lineages.
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影响因子：
1.7
作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
Walichiewicz, J
影响因子：
3.1
作者：
Broad, TE;Forrest, JW;Scott, IAS
通讯作者：
Scott, IAS
影响因子：
1.6
作者：
Bernatkova, Anna;Oyunsaikhan, Ganbaatar;Simek, Jaroslav;Komarkova, Martina;Bobek, Miroslav;Ceacero, Francisco
通讯作者：
Ceacero, Francisco
影响因子：
10.7
作者：
Cappelletti, Eleonora;Piras, Francesca M.;Sola, Lorenzo;Santagostino, Marco;Abdelgadir, Wasma A.;Raimondi, Elena;Lescai, Francesco;Nergadze, Solomon G.;Giulotto, Elena
通讯作者：
Giulotto, Elena
影响因子：
4
作者：
Kerekes, Viola;Sandor, Istvan;Barta, Zoltan
通讯作者：
Barta, Zoltan