Incorporating functionalized polyethylene glycol lipids into reprecipitated conjugated polymer nanoparticles for bioconjugation and targeted labeling of cells.
Incorporating functionalized polyethylene glycol lipids into reprecipitated conjugated polymer nanoparticles for bioconjugation and targeted labeling of cells.
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DOI：
10.1039/c0nr00746c
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发表时间：
2011-03
期刊：
影响因子：
6.7
通讯作者：
Christensen KA
中科院分区：
文献类型：
--
作者：
Kandel PK;Fernando LP;Ackroyd PC;Christensen KA
我们报告了一种简单而快速的方法来制备非常明亮的，功能化的，稳定的，和生物相容性的共轭聚合物纳米粒子纳入功能化的聚乙二醇（PEG）脂质再沉淀。这些新的纳米颗粒保留了无PEG脂质制备的共轭聚合物纳米颗粒的基本光谱性质，但与未改性的共轭聚合物纳米颗粒相比，表现出更大的亲水性和量子产率。通过TEM测定，这些混合纳米颗粒的尺寸为21-26 nm。值得注意的是，这些纳米颗粒制备有几个PEG脂质官能端基，并且生物素和羧基部分可以容易地生物缀合。我们已经证明了使用生物素PEG脂质缀合的聚合物纳米颗粒与链霉亲和素的相互作用，这些末端基团用于官能化的可用性。生物素化PEG脂质缀合的聚合物纳米颗粒结合链霉亲和素连接的磁珠，而羧基和甲氧基PEG脂质修饰的纳米颗粒没有。类似地，生物素化PEG脂质缀合的聚合物纳米颗粒结合链霉亲和素包被的载玻片，并且可以被可视化为衍射限制点，而不含PEG脂质或具有非生物素PEG脂质端基的纳米颗粒不结合。为了证明纳米颗粒功能化可用于特异性细胞蛋白的靶向标记，使用链霉亲和素作为夹心形式的接头，将生物素化的PEG脂质缀合的聚合物纳米颗粒与J774A.1细胞表面受体上的生物素化的抗CD 16/32抗体结合。这些数据证明了这些新的纳米颗粒用于基于荧光的成像和传感的实用性。我们报告了一种简单快速的再沉淀方法，以制备非常明亮的，功能化的，生物相容性的共轭聚合物纳米粒子（CPNs）纳入功能化的聚乙二醇（PEG）脂质。这些21-25 nm的CPN显示出比未改性的CPN更大的亲水性和量子产率，并且在溶液中高度稳定。我们证明了生物共轭和有针对性的交付抗体染色细胞使用生物素功能化的PEG脂质PFBT纳米粒子。
We report a simple and rapid method to prepare extremely bright, functionalized, stable, and biocompatible conjugated polymer nanoparticles incorporating functionalized polyethylene glycol (PEG) lipids by reprecipitation. These new nanoparticles retain the fundamental spectroscopic properties of conjugated polymer nanoparticles prepared without PEG lipid, but demonstrate greater hydrophilicity and quantum yield compared to unmodified conjugated polymer nanoparticles. The sizes of these hybrid nanoparticles, as determined by TEM, were 21–26 nm. Notably, these nanoparticles were prepared with several PEG lipid functional end groups and the biotin and carboxy moieties can be easily bioconjugated. We have demonstrated the availability of these end groups for functionalization using the interaction of biotin PEG lipid conjugated polymer nanoparticles with streptavidin. Biotinylated PEG lipid conjugated polymer nanoparticles bound streptavidin-linked magnetic beads, while carboxy and methoxy PEG lipid modified nanoparticles did not. Similarly, biotinylated PEG lipid conjugated polymer nanoparticles bound streptavidin-coated glass slides and could be visualized as diffraction-limited spots, while nanoparticles without PEG lipid or with non-biotin PEG lipid end groups were not bound. To demonstrate that nanoparticle functionalization could be used for targeted labeling of specific cellular proteins, biotinylated PEG lipid conjugated polymer nanoparticles were bound to biotinylated anti-CD16/32 antibodies on J774A.1 cell surface receptors, using streptavidin as a linker in a sandwich format. These data demonstrate the utility of these new nanoparticles for fluorescence based imaging and sensing. We report a simple and rapid reprecipitation method to prepare extremely bright, functionalized, and biocompatible conjugated polymer nanoparticles (CPNs) incorporating functionalized polyethylene glycol (PEG) lipids. These 21–25 nm CPNs demonstrate greater hydrophilicity and quantum yield than unmodified CPNs and are highly stable in solution. We demonstrate bioconjugation and targeted delivery to antibody stained cells using biotin-functionalized PEG lipid PFBT nanoparticles.
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