Phenotypic changes in cultured smooth muscle cells: limitation or opportunity for tissue engineering of hollow organs?
Phenotypic changes in cultured smooth muscle cells: limitation or opportunity for tissue engineering of hollow organs?
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DOI：
10.1002/term.451
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发表时间：
2012-07
影响因子：
3.3
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Huber, Alexander;Badylak, Stephen F.
平滑肌细胞（SMCs）通常被用作传统组织工程技术重建中空器官的细胞来源。然而，使用组织特异性SMCs的必要性和优势尚不清楚。本研究研究了从主动脉、食道和膀胱三种不同组织中分离的大鼠SMC群体在分离和体外扩增后发生的表型变化。rSMCs通过酶分散分离，并通过常规细胞培养技术扩增，得到微观均匀的群体。根据前两代标记蛋白的表达监测SMC表型。三个SMC群体中的两个（rSMC-a和rSMC-e）在前两次传代中表现出标记蛋白谱的显著变化，导致了既不完全收缩也不完全合成的同质表型。膀胱的SMCs则没有这种变化。观察到三个rSMC群体之间在增殖活性和基因表达模式方面的差异，表明保留了一些组织特异性细胞特征。总之，SMCs的表型变化是传统细胞分离和扩增技术的结果，因此质疑再生医学应用中组织特异性细胞来源的必要性。
Smooth muscle cells (SMCs) are typically used as a cell source for the reconstruction of hollow organs by conventional tissue engineering techniques. However, the necessity and advantage for the use of tissue specific SMCs is unknown. The present study investigated the phenotypic changes that occur following isolation and in vitro expansion of rat SMC populations isolated from three different tissues: the aorta, esophagus, and urinary bladder. rSMCs were isolated by enzymatic dispersion and expanded by conventional cell culture techniques yielding microscopically homogeneous populations. SMC phenotypes were monitored according to their expression of marker proteins during the first two passages. Two of the three SMC populations (rSMC-a and rSMC-e) showed a marked change in their marker protein profiles during the first two passages which resulted in a homogenous phenotype that was neither fully contractile nor fully synthetic. SMCs from the urinary bladder did not show such a shift. Differences between the three rSMC populations were observed with regard to proliferative activity and gene expression patterns suggesting the retention of some tissue-specific cell characteristics. In summary, phenotypic changes in SMCs occur as a result of conventional cell isolation and expansion techniques, thus questioning the necessity of a tissue specific cell source for regenerative medicine applications.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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DOI：
10.1513/pats.200802-022aw
发表时间：
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期刊：
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