Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant
Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant
复制标题
固氮根瘤菌 c-di-GMP 磷酸二酯酶 Chp1 参与宿主植物的运动、EPS 生产和结瘤
DOI：
10.1007/s00253-020-10404-6
复制
发表时间：
2020-01
影响因子：
5
通讯作者：
Zhihong Xie
中科院分区：
文献类型：
--
作者：
Yu Sun;Yanan Liu;Xiaolin Liu;Xiaoxiao Dang;Xiaoyan Dong;Zhihong Xie
Establishment of the rhizobia-legume symbiosis is usually accompanied by hydrogen peroxide (H2O2) production by the legume host at the site of infection, a process detrimental to rhizobia. In Azorhizobium caulinodans ORS571, deletion of chp1, a gene encoding c-di-GMP phosphodiesterase, led to increased resistance against H2O2and to elevated nodulation efficiency on its legume host Sesbania rostrata. Three domains were identified in the Chp1: a PAS domain, a degenerate GGDEF domain, and an EAL domain. An in vitro enzymatic activity assay showed that the degenerate GGDEF domain of Chp1 did not have diguanylate cyclase activity. The phosphodiesterase activity of Chp1 was attributed to its EAL domain which could hydrolyse c-di-GMP into pGpG. The PAS domain functioned as a regulatory domain by sensing oxygen. Deletion of Chp1 resulted in increased intracellular c-di-GMP level, decreased motility, increased aggregation, and increased EPS (extracellular polysaccharide) production. H2O2-sensitivity assay showed that increased EPS production could provide ORS571 with resistance against H2O2. Thus, the elevated nodulation efficiency of the ∆chp1 mutant could be correlated with a protective role of EPS in the nodulation process. These data suggest that c-di-GMP may modulate the A. caulinodans-S. rostrata nodulation process by regulating the production of EPS which could protect rhizobia against H2O2.
登录
查看更多内容
影响因子：
5.8
作者：
Chua SL;Ding Y;Liu Y;Cai Z;Zhou J;Swarup S;Drautz-Moses DI;Schuster SC;Kjelleberg S;Givskov M;Yang L
通讯作者：
Yang L
影响因子：
16
作者：
Paul K;Nieto V;Carlquist WC;Blair DF;Harshey RM
通讯作者：
Harshey RM
细菌外多糖产生所需的环状-DI-GMP受体。
影响因子：
3.6
作者：
Lee, Vincent T;Matewish, Jody M;Kessler, Jennifer L;Hyodo, Mamoru;Hayakawa, Yoshihiro;Lory, Stephen
通讯作者：
Lory, Stephen
影响因子：
3.2
作者：
GOETHALS, K;LEYMAN, B;HOLSTERS, M
通讯作者：
HOLSTERS, M
DOI：
--
发表时间：
2002
期刊：
Nihon eiseigaku zasshi. Japanese journal of hygiene
影响因子：
--
作者：
E. Sato;M. Inoue
通讯作者：
E. Sato;M. Inoue