A microfluidic localized, multiple cell culture array using vacuum actuated cell seeding: integrated anticancer drug testing.
A microfluidic localized, multiple cell culture array using vacuum actuated cell seeding: integrated anticancer drug testing.
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DOI：
10.1007/s10544-013-9779-3
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发表时间：
2013-12
影响因子：
2.8
通讯作者：
Pappas, Dimitri
中科院分区：
文献类型：
--
作者：
Gao, Yan;Li, Peng;Pappas, Dimitri
在这项研究中，我们介绍了一种新颖而方便的方法，利用一步真空驱动在微流控室的局部阵列中培养多个细胞。在一个设备中，我们集成了8个单独可寻址的培养室区域，每个区域只需要一个简单的真空操作来播种细胞系。采用高纯度（99.9%-100%）序贯注射方式，将4株细胞系接种于同一装置指定区域，长期培养。HuT - 78、Ramos、PC-3和C166-GFP细胞片上同时培养48 h，存活率分别为92%+/−2%、94%+/−4%、96%+/−2%和97%+/−2%。本实验C166-GFP细胞的最长培养时间为168 h，存活率为96%+/−10%。每个单独侧通道的细胞增殖可以被跟踪。通过扩散和荧光素溶液研究了主通道和侧通道之间的质量传递。该设备的主要优点是能够在同一设备上进行多种基于细胞的分析，以便更好地进行比较研究。staurosporine或抗人CD95作用细胞16 h后，HuT 78、CCRF-CEM、PC-3和Ramos细胞的凋亡细胞率分别为staurosporine处理后的36%+/ - 3%、24%+/ - 4%、12%+/ - 2%、18%+/ - 4%，抗人CD95处理后的凋亡细胞率分别为63%+/ - 2%、45%+/ - 1%、3%+/ - 3%、27%+/ - 12%。具有增强的集成度，易于使用和制造以及灵活性的优点，该设备将适用于长期的多细胞监测和基于细胞的分析。
In this study, we introduced a novel and convenient approach to culture multiple cells in localized arrays of microfluidic chambers using one-step vacuum actuation. In one device, we integrated 8 individually addressable regions of culture chambers, each only requiring one simple vacuum operation to seed cells lines. Four cell lines were seeded in designated regions in one device via sequential injection with high purity (99.9%-100%) and cultured for long-term. The on-chip simultaneous culture of HuT 78, Ramos, PC-3 and C166-GFP cells for 48 h was demonstrated with viabilities of 92%+/−2%, 94%+/−4%, 96%+/−2% and 97%+/−2%, respectively. The longest culture period for C166-GFP cells in this study was 168 h with a viability of 96%+/−10%. Cell proliferation in each individual side channel can be tracked. Mass transport between the main channel and side channels was achieved through diffusion and studied using fluorescein solution. The main advantage of this device is the capability to perform multiple cell-based assays on the same device for better comparative studies. After treating cells with staurosporine or anti-human CD95 for 16 h, the apoptotic cell percentage of HuT 78, CCRF-CEM, PC-3 and Ramos cells were 36%+/−3%, 24%+/−4%, 12%+/−2%, 18%+/−4% for staurosporine, and 63%+/−2%, 45%+/−1%, 3%+/−3%, 27%+/−12% for anti-human CD95, respectively. With the advantages of enhanced integration, ease of use and fabrication, and flexibility, this device will be suitable for long-term multiple cell monitoring and cell based assays.
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DOI：
10.1039/c0an00845a
发表时间：
2011-09-07
期刊：
The Analyst
影响因子：
--
作者：
Khanal G;Chung K;Solis-Wever X;Johnson B;Pappas D
通讯作者：
Pappas D
影响因子：
6.1
作者：
Yu, Hongmei;Alexander, Caroline M.;Beebe, David J.
通讯作者：
Beebe, David J.
影响因子：
3.9
作者：
Liu, Ke;Pitchimani, Rajasekar;Pappas, Dimitri
通讯作者：
Pappas, Dimitri
影响因子：
56.9
作者：
Unger, MA;Chou, HP;Quake, SR
通讯作者：
Quake, SR
影响因子：
6.1
作者：
O'Neill, Adrian T.;Monteiro-Riviere, Nancy A.;Walker, Glenn M.
通讯作者：
Walker, Glenn M.