Focal high cell density generates a gradient of patterns in self-organizing vascular mesenchymal cells.
Focal high cell density generates a gradient of patterns in self-organizing vascular mesenchymal cells.
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DOI：
10.1159/000339568
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发表时间：
2012
影响因子：
1.7
通讯作者：
Demer LL
中科院分区：
文献类型：
--
作者：
Cheng H;Reddy A;Sage A;Lu J;Garfinkel A;Tintut Y;Demer LL
在胚胎发生中，结构模式，如血管分支，可以通过反应扩散机制形成，其中激活剂和抑制剂形态发生剂引导细胞形成周期性聚集体。我们以前发现，血管间充质细胞（VMC）自发聚集成结节状结构和形态对调节聚集成斑点和条纹的模式。为了测试活化剂形态发生的焦点变化对VMC模式形成的影响，我们通过在融合单层上的克隆环内接种第二层VMC来创建高细胞密度的焦点区。24小时后，移除环，并通过相差显微镜监测图案形成。在第2-8天，图案从均匀分布发展为漩涡、螺旋状和斑点图案。在局灶性高密度区和狭窄的晕圈区内，细胞聚集成斑点图案;在平板的最外侧区，细胞形成一种网状图案。聚集体所占的面积显着大于在HDZ或晕的最外层区域。HDZ内的图案进展速率作为其电镀密度的函数而增加。因此，细胞密度的局灶性差异可以驱动组织结构中的图案形成梯度，例如血管分支。
In embryogenesis, structural patterns, such as vascular branching, may form via a reaction-diffusion mechanism in which activator and inhibitor morphogens guide cells into periodic aggregates. We previously found that vascular mesenchymal cells (VMC) spontaneously aggregate into nodular structures and that morphogen pairs regulate the aggregation into patterns of spots and stripes. To test the effect of a focal change in activator morphogen on VMC pattern formation, we created a focal zone of high cell density by plating a second layer VMC within a cloning ring over a confluent monolayer. After 24 hours, the ring was removed, and pattern formation monitored by phase-contrast microscopy. At days 2–8, the patterns progressed from uniform distributions to swirl, labyrinthine, and spot patterns. Within the focal high-density zone and a narrow halo zone, cells aggregated into spot patterns; in the outermost zone of the plate, cells formed a labyrinthine pattern. Area occupied by aggregates was significantly greater in the outermost zone than in the HDZ or halo. The rate of pattern progression within the HDZ increased as a function of its plating density. Thus, focal differences in cell density may drive pattern formation gradients in tissue architecture, such as vascular branching.
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影响因子：
2
作者：
MURRAY, JD
通讯作者：
MURRAY, JD
影响因子：
1.6
作者：
NAGORCKA, BN
通讯作者：
NAGORCKA, BN
DOI：
10.1098/rstb.1952.0012
发表时间：
1952-01-01
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
TURING, AM
通讯作者：
TURING, AM
DOI：
10.1073/pnas.0308436101
发表时间：
2004-06-22
影响因子：
11.1
作者：
Garfinkel, A;Tintut, Y;Demer, LL
通讯作者：
Demer, LL
DOI：
10.1006/bbrc.2001.5351
发表时间：
2001-08-10
影响因子：
3.1
作者：
Ghosh-Choudhury, N;Choudhury, GG;Harris, SE
通讯作者：
Harris, SE