The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells.
The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells.
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DOI：
10.3390/ijms150814026
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发表时间：
2014-08-13
影响因子：
5.6
通讯作者：
Umeda M
中科院分区：
文献类型：
--
作者：
Katayama N;Kato H;Taguchi Y;Tanaka A;Umeda M
牙釉质基质衍生物广泛应用于牙周组织再生治疗。然而，由于EMD的生物活性因批次不同而不同，使用合成肽可以避免使用动物来源，因此包含EMD活性成分的完全合成多肽(SP)将是有用的。在这项研究中，从EMD合成的寡肽是否有助于牙周组织的再生。我们研究了SP对参与组织再生的人骨髓间充质干细胞(MSCs)增殖和成骨细胞分化的影响。将SP(0~1000 ng/mL)作用于MSCs，确定最适浓度。我们观察了SP对细胞增殖和成骨分化指标，如碱性磷酸酶活性、1型前胶原C肽和骨钙素的产生以及对矿化的影响。此外，我们还研究了细胞外信号相关蛋白(ERK)在SP诱导的细胞增殖和成骨细胞分化中的作用。我们的结果表明，SP促进了人MSCs的这些过程，而ERK抑制剂抑制了这些作用。综上所述，SP可能通过ERK途径促进人MSCs的细胞增殖和成骨分化。
Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.
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影响因子：
7.6
作者：
Iwata, T;Morotome, Y;Oida, S
通讯作者：
Oida, S
影响因子：
4.3
作者：
Jue, Seong-Suk;Lee, Won Young;Lee, Baeksoo
通讯作者：
Lee, Baeksoo
影响因子：
7.6
作者：
Keila, S;Nemcovsky, CE;Weinreb, M
通讯作者：
Weinreb, M
DOI：
10.1016/j.tripleo.2004.02.063
发表时间：
2004-08-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Hägewald, S;Pischon, N;Zimmermann, B
通讯作者：
Zimmermann, B
影响因子：
8.5
作者：
Cheng, L.;Lin, Z. K.;Tian, L.
通讯作者：
Tian, L.