Expression of MUC1 mucins inversely correlated with post-surgical survival of renal cell carcinoma patients.
Expression of MUC1 mucins inversely correlated with post-surgical survival of renal cell carcinoma patients.
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MUC1粘蛋白的表达与肾细胞癌患者的术后生存呈负相关。
DOI：
10.1038/sj.bjc.6690355
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发表时间：
1999-04
影响因子：
8.8
通讯作者：
Irimura, T
中科院分区：
文献类型：
--
作者：
Fujita, K;Denda, K;Yamamoto, M;Matsumoto, T;Fujime, M;Irimura, T
用单抗MY.1E12检测了不同进展阶段和不同组织分级的正常肾脏和肾细胞癌(RCC)手术标本中含唾液酸化碳水化合物(唾液酸化的粘蛋白)的MUC1粘蛋白的表达。免疫组织化学研究表明，该抗体的结合部位位于远曲小管、Henle‘s环和集合管上皮细胞的顶侧。然而，近端曲管，被认为是肾癌起源的地方，没有染色。这种抗体还与进展晚期和转移部位的肾细胞癌以及组织学高级别(未分化)的肾细胞癌强烈结合。该表位可能是唾液酸化的MUC1粘蛋白，不仅沿细胞膜表面检测到，而且在细胞质中也检测到。唾液酸化MUC1粘蛋白的表达水平与肾癌患者的生存期和根治术后无瘤生存期呈负相关。Western印迹分析表明，肾细胞癌唾液酸化的MUC1粘蛋白的电泳率高于正常肾组织。提示唾液酸化的MUC1粘蛋白在某些人肾细胞癌群体中的高水平表达与疾病的侵袭性有关，例如形成转移的倾向。©1999癌症研究活动
Surgical specimens of the normal kidney and of renal cell carcinoma (RCC) tissues at different stages of progression and of various histological grades were examined for the expression of MUC1 mucins with sialylated carbohydrates (sialylated MUC1 mucins) using a monoclonal antibody MY.1E12. Immunohistochemical studies revealed that the binding sites for this antibody were localized to the apical side of the epithelial cells of the distal convoluted tubules, Henle's loops and collecting ducts. However, proximal convoluted tubules, where RCC is considered to originate, were not stained. This antibody also bound strongly to RCC at advanced stages of progression and at metastatic sites, and to RCC of histologically high grades (undifferentiated). The epitope, presumably sialylated MUC1 mucin, was detected not only along the surface of the cell membranes but also in the cytoplasm. The level of expression of sialylated MUC1 mucins was inversely correlated with the survival of the patients with RCC and the disease-free survival period after curative surgery. Western blot analysis demonstrated that the electrophoretic mobility of sialylated MUC1 mucins of RCC was greater than that from the normal kidney. It is suggested that high levels of expression of sialylated MUC1 mucins in certain human RCC populations correlate with the aggressiveness of the disease, such as the tendency to form metastasis. © 1999 Cancer Research Campaign
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DOI：
10.1016/0304-419x(94)90002-7
发表时间：
1994-05-27
影响因子：
11.2
作者：
PONTA, H;SLEEMAN, J;HERRLICH, P
通讯作者：
HERRLICH, P
影响因子：
8.8
作者：
KATAGIRI, A;WATANABE, R;TOMITA, Y
通讯作者：
TOMITA, Y
影响因子：
82.9
作者：
Gimmi, CD;Morrison, BW;Nadler, LM
通讯作者：
Nadler, LM
影响因子：
29.4
作者：
NAKAMORI, S;OTA, DM;IRIMURA, T
通讯作者：
IRIMURA, T
影响因子：
1
作者：
MACKAY, B;ORDONEZ, NG;BENNINGTON, JL
通讯作者：
BENNINGTON, JL