Substantivity of chlorhexidine to human dentin.
Substantivity of chlorhexidine to human dentin.
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DOI：
10.1016/j.dental.2010.04.002
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发表时间：
2010-08
期刊：
影响因子：
5
通讯作者：
Pashley, David H.
中科院分区：
文献类型：
--
作者：
Carrilho, Marcela R.;Carvalho, Ricardo M.;Sousa, Ethan N.;Nicolau, Jose;Breschi, Lorenzo;Mazzoni, Annalisa;Tjaderhane, Leo;Tay, Franklin R.;Agee, Kelli;Pashley, David H.
为了更好地理解CHX在树脂-牙本质键保存中的作用，本研究调查了CHX对人牙本质的实质性。牙本质盘（n= 45）取自人类第三磨牙中冠状部分。三分之一的牙本质盘保持矿化（MD），其余三分之二的牙本质盘采用37%磷酸15 s部分脱矿（PDD）或10%磷酸15 s完全脱矿（TDD）。制备了羟基磷灰石（HA）圆盘。分别用1)10 μL蒸馏水（对照）、2)10 μL 0.2%二乙酸氯己定（0.2% CHX）或3)10 μL 2%二乙酸氯己定（2% CHX）处理。然后用1ml PBS （pH 7.4, 37°C）孵育。在孵育0.5h、1h、3h、6h、24h、168h （1wk）、672h （4wk）和1344h （8wk）后，以chx给药剂量的函数评价其实质性。用分光光度法测定洗脱液中CHX的浓度。牙本质底物（MD、PPD或TDD）中仍保留了大量CHX，与CHX施加剂量或孵育时间无关（p<0.05）。仅在CHX浓度最高（2%）的标本上观察到大量CHX残留在HA上（p<0.05）。CHX对牙本质的突出实质及其对牙本质蛋白酶的抑制作用可能解释了为什么CHX可以延长树脂-牙本质键的持久性。
To better comprehend the role of CHX in the preservation of resin-dentin bonds, this study investigated the substantivity of CHX to human dentin. Dentin disks (n= 45) were obtained from the mid-coronal portion of human third molars. One-third of dentin disks were kept mineralized (MD), while the other two-thirds had one of the surfaces partially demineralized with 37% phosphoric acid for 15 s (PDD) or they were totally demineralized with 10% phosphoric acid (TDD). Disks of hydroxyapatite (HA) were also prepared. Specimens were treated with: 1) 10 μL of distilled water (controls), 2) 10 μL of 0.2% chlorhexidine diacetate (0.2% CHX) or 3) 10 μL of 2% chlorhexidine diacetate (2% CHX). Then, they were incubated in 1 mL of PBS (pH 7.4, 37 °C). Substantivity was evaluated as a function of the CHX-applied dose after: 0.5h, 1h, 3h, 6h, 24h, 168h (1wk), 672h (4wks) and 1344h (8wks) of incubation. CHX concentration in eluates was spectrophotometrically analyzed at 260 nm. Significant amounts of CHX remained retained in dentin substrates (MD, PPD or TDD), independent on the CHX-applied dose or time of incubation (p<0.05). High amounts of retained CHX onto HA were observed only for specimens treated with the highest concentration of CHX (2%) (p<0.05). The outstanding substantivity of CHX to dentin and its reported effect on the inhibition of dentinal proteases may explain why CHX can prolong the durability of resin-dentin bonds.
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影响因子：
3.3
作者：
Blackburn, Richard S.;Harvey, Anna;Russell, Stephen J.
通讯作者：
Russell, Stephen J.
影响因子：
7.6
作者：
Carrilho, M. R. O.;Carvalho, R. M.;Tjaderhane, L.
通讯作者：
Tjaderhane, L.
DOI：
10.1111/j.1365-263x.2008.00914.x
发表时间：
2008-11-01
影响因子：
3.8
作者：
De Amorim, Rodrigo Guedes;Leal, Soraya Coelho;De Toledo, Orlando Ayrton
通讯作者：
De Toledo, Orlando Ayrton
DOI：
10.1002/jbm.b.31295
发表时间：
2009-07
影响因子：
3.4
作者：
Carrilho, Marcela R.;Tay, Franklin R.;Donnelly, Adam M.;Agee, Kelli A.;Tjaederhane, Leo;Mazzoni, Annalisa;Breschi, Lorenzo;Foulger, Stephen;Pashley, David H.
通讯作者：
Pashley, David H.
影响因子：
2.2
作者：
Brackett, M. G.;Tay, F. R.;Pashley, D. H.
通讯作者：
Pashley, D. H.