Identification and characterisation of resistance gene analogues from wild Chinese Vitis species
Identification and characterisation of resistance gene analogues from wild Chinese Vitis species
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中国野生葡萄品种抗性基因类似物的鉴定和表征
DOI：
10.1080/14620316.2008.11512390
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发表时间：
2008-01
影响因子：
1.9
通讯作者：
王跃进
中科院分区：
文献类型：
--
作者：
王跃进
抗性基因类似物（RGAs）的收集是分离植物抗病基因的第一步。利用已克隆的植物抗性基因核苷酸结合位点亮氨酸丰富重复序列（NBS-LRR）结构域的两个保守基序，设计了退化寡核苷酸引物，并应用于5种野生中国葡萄品种（V. quinquangularis ‘ 83-4-96 ’、V. bashanica ‘ Xunyang-8 ’、V. davidii ‘ Tangwei ’、V. yeshanensis ‘ Yanshan ’和V. bryoniifolia ' Anlin-3 '）的RGAs分离。共获得55个具有不间断开放阅读框的RGAs，并在每个物种中进一步组装成26个不同的RGAs。这些RGAs被划分为6个不同的科。他们推断的氨基酸序列包含NBS-LRR抗性基因的保守基序特征。然而，所鉴定的RGAs序列差异很大。非同义与同义替换的比例较低，且家族内未检测到重组事件，表明葡萄RGAs NBS区域处于纯化选择而非多样化选择。此外，根据与其他已知植物R基因的系统发育分析，本研究中鉴定的所有RGAs都被归类为NBS-LRR抗性基因的非toll /白细胞介素1受体（TIR）型。
Summary Generating a collection of resistance gene analogues (RGAs) is an initial step towards the isolation of plant disease resistance genes. Based on the two conserved motifs in the nucleotide-binding site leucine-rich repeat (NBS-LRR) domain from previously cloned plant resistance genes, degenerate oligonucleotide primers were designed and used to isolate RGAs from five wild Chinese Vitis species: V. quinquangularis ‘83-4-96’, V. bashanica ‘Xunyang-8’, V. davidii ‘Tangwei’, V. yeshanensis ‘Yanshan’, and V. bryoniifolia ‘Anlin-3’. A total of 55 RGAs with uninterrupted open reading frames were obtained and further assembled within each species into 26 distinct RGAs. These RGAs were classified into six distinct families. Their deduced amino acid sequences contained the conserved motifs characteristic of NBS-LRR resistance genes. However, the sequences of the identified RGAs were very divergent from each other. The low ratio of non-synonymous to synonymous substitutions, and non-detectable recombination events within families, indicate that the NBS regions of Vitis RGAs are under purifying selection rather than diversifying selection. Furthermore, all the RGAs identified in this study were grouped into the non-Toll/Interleukin 1 receptor (TIR)-type of NBS-LRR resistance genes, based on phylogenetic analysis with other known plant R genes.
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影响因子：
5.4
作者：
Jose Miguel Soriano;Santiago Vilanova;C. Romero;G. Llácer;M. Badenes
通讯作者：
Jose Miguel Soriano;Santiago Vilanova;C. Romero;G. Llácer;M. Badenes
DOI：
10.1073/pnas.93.21.11746
发表时间：
1996-10-15
影响因子：
11.1
作者：
Kanazin, V;Marek, LF;Shoemaker, RC
通讯作者：
Shoemaker, RC
影响因子：
5.2
作者：
X. Pei;Sheng-Hui Li;Ying Jiang;Yongqiang Zhang;Zhi-Xing Wang;S. Jia
通讯作者：
X. Pei;Sheng-Hui Li;Ying Jiang;Yongqiang Zhang;Zhi-Xing Wang;S. Jia
影响因子：
10.7
作者：
M. Nei;T. Gojobori
通讯作者：
M. Nei;T. Gojobori
DOI：
--
发表时间：
2001-01
期刊：
--
影响因子：
--
作者：
J. Sambrook;E. Fritsch;T. Maniatis
通讯作者：
J. Sambrook;E. Fritsch;T. Maniatis