Tuning backbones and side-chains of cationic conjugated polymers for optical signal amplification of fluorescent DNA detection.
Tuning backbones and side-chains of cationic conjugated polymers for optical signal amplification of fluorescent DNA detection.
复制标题
调整阳离子共轭聚合物的主链和侧链，用于荧光 DNA 检测的光学信号放大。
DOI：
10.1016/j.bios.2009.03.003
复制
发表时间：
2009-06
影响因子：
12.6
通讯作者：
中科院分区：
文献类型：
--
作者：
采用三种具有不同主链几何形状和电荷密度的阳离子共轭聚合物（CCPs），研究了它们的共轭主链和侧链性质以及DNA两亲性的转变如何在CCP/DNA- c * （DNA- c *：荧光团标记的DNA）配合物中相互作用，从而影响基于Förster共振能量转移（FRET）的荧光DNA检测的光信号放大。通过检测每个CCP对dsDNA- c * （dsDNA：双链DNA）和ssDNA- c * （ssDNA：单链DNA）的FRET效率，证明了扭曲共轭骨架和更高的电荷密度有助于CCP/dsDNA- c *配合物的静电吸引，并提高了对DNA杂交的敏感性。特别是使用具有扭曲共轭主链和最高电荷密度的CCP，发现FRET对dsDNA- c *的效率比对ssDNA- c *的效率高7倍以上，表明对dsDNA和ssDNA具有较高的信号放大能力。相反，线性共轭骨架和较低的电荷密度有利于CCP/ssDNA-C*配合物的疏水相互作用。这些发现为新型敏感CCP的设计提供了指导，它可以用于识别许多其他重要的DNA活动，包括DNA两亲性的转变，如DNA杂交，如特定的DNA与离子结合，DNA的一些二级或三级结构变化，等等。
Three cationic conjugated polymers (CCPs) exhibiting different backbone geometries and charge densities were used to investigate how their conjugated backbone and side chain properties, together with the transitions of DNA amphiphilic properties, interplay in the CCP/DNA-C* (DNA-C*: fluorophore-labeled DNA) complexes to influence the optical signal amplification of fluorescent DNA detection based on Förster resonance energy transfer (FRET). By examining the FRET efficiencies to dsDNA-C* (dsDNA: double-stranded DNA) and ssDNA-C* (ssDNA: single-stranded DNA) for each CCP, twisted conjugated backbones and higher charge densities were proved to facilitate electrostatic attraction in CCP/dsDNA-C* complexes, and induced improved sensitivity to DNA hybridization. Especially, by using the CCP with twisted conjugated backbone and the highest charge density, a more than 7-fold higher efficiency of FRET to dsDNA-C* was found than to ssDNA-C*, indicating a high signal amplification for discriminating between dsDNA and ssDNA. By contrast, linear conjugated backbones and lower charge density were demonstrated to favor hydrophobic interactions in CCP/ssDNA-C* complexes. These findings provided guidelines for the design of novel sensitive CCP, which can be useful to recognize many other important DNA activities involving transitions of DNA amphiphilic properties like DNA hybridization, such as specific DNA binding with ions, some secondary or tertiary structural changes of DNA, and so forth.
登录
查看更多内容
影响因子：
7.4
作者：
Ahmed Najari;H. Ho;J. Gravel;Philippe Nobert;D. Boudreau;M. Leclerc
通讯作者：
Ahmed Najari;H. Ho;J. Gravel;Philippe Nobert;D. Boudreau;M. Leclerc
DOI：
10.1073/pnas.162375999
发表时间：
2002-08-20
影响因子：
11.1
作者：
Gaylord, BS;Heeger, AJ;Bazan, GC
通讯作者：
Bazan, GC
影响因子：
15
作者：
He, F;Tang, YL;Zhu, DB
通讯作者：
Zhu, DB
影响因子：
15
作者：
Ho, HA;Doré, K;Leclerc, M
通讯作者：
Leclerc, M
影响因子：
2.7
作者：
M. Berberan-Santos;J. Martinho
通讯作者：
M. Berberan-Santos;J. Martinho