Engineered whole cut meat-like tissue by the assembly of cell fibers using tendon-gel integrated bioprinting.
Engineered whole cut meat-like tissue by the assembly of cell fibers using tendon-gel integrated bioprinting.
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DOI：
10.1038/s41467-021-25236-9
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发表时间：
2021-08-24
影响因子：
16.6
通讯作者：
Matsusaki M
中科院分区：
文献类型：
--
作者：
Kang DH;Louis F;Liu H;Shimoda H;Nishiyama Y;Nozawa H;Kakitani M;Takagi D;Kasa D;Nagamori E;Irie S;Kitano S;Matsusaki M
随着目前对培养肉的兴趣，哺乳动物细胞肉大多是非结构化的。因此，对人造牛排类肉类的需求仍然很高。我们证明了在体外构建的工程牛排样组织组装的三种类型的牛细胞纤维（肌肉，脂肪和血管）。由于实际的肉是连接到肌腱的纤维的对齐组装，用于收缩和松弛的动作，因此开发了肌腱-凝胶集成生物打印来构建肌腱样凝胶。在这项研究中，总共72个纤维，包括42个肌肉，28个脂肪组织和2个毛细血管，通过肌腱凝胶集成生物打印和手动组装来制造直径为5 mm，长度为10 mm的牛排状肉。这里开发的肌腱-凝胶集成生物打印技术可能是一种很有前途的技术，用于制造所需类型的牛排样培养肉类。基于哺乳动物细胞的培养肉大多是非结构化的，因此对人造牛排肉的需求很大。在这里，作者提出了一个组装牛排样组织的牛骨骼肌，脂肪组织，毛细血管组织制造的肌腱凝胶集成打印技术。
With the current interest in cultured meat, mammalian cell-based meat has mostly been unstructured. There is thus still a high demand for artificial steak-like meat. We demonstrate in vitro construction of engineered steak-like tissue assembled of three types of bovine cell fibers (muscle, fat, and vessel). Because actual meat is an aligned assembly of the fibers connected to the tendon for the actions of contraction and relaxation, tendon-gel integrated bioprinting was developed to construct tendon-like gels. In this study, a total of 72 fibers comprising 42 muscles, 28 adipose tissues, and 2 blood capillaries were constructed by tendon-gel integrated bioprinting and manually assembled to fabricate steak-like meat with a diameter of 5 mm and a length of 10 mm inspired by a meat cut. The developed tendon-gel integrated bioprinting here could be a promising technology for the fabrication of the desired types of steak-like cultured meats. Mammalian cell-based cultured meat has mostly been unstructured, leaving a demand for artificial steak-like meat. Here the authors present an assembled steak-like tissue of bovine skeletal muscle, adipose tissue, and blood capillary tissue fabricated by tendon-gel integrated printing technology.
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影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
肌肉生长抑制素在哺乳动物脂肪量调节中的作用
影响因子：
4.5
作者：
Deng B;Zhang F;Wen J;Ye S;Wang L;Yang Y;Gong P;Jiang S
通讯作者：
Jiang S
影响因子：
9.7
作者：
Heher, Philipp;Maleiner, Babette;Fuchs, Christiane
通讯作者：
Fuchs, Christiane
影响因子：
2.5
作者：
GUERIN, CW;HOLLAND, PC
通讯作者：
HOLLAND, PC
影响因子：
14
作者：
Costantini, Marco;Testa, Stefano;Gargioli, Cesare
通讯作者：
Gargioli, Cesare