Carbohydrate stress affecting fruitlet abscission and expression of genes related to auxin signal transduction pathway in litchi.
Carbohydrate stress affecting fruitlet abscission and expression of genes related to auxin signal transduction pathway in litchi.
复制标题
碳水化合物胁迫对荔枝果实脱落及生长素信号转导通路相关基因表达的影响
DOI：
10.3390/ijms131216084
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发表时间：
2012-11-29
影响因子：
5.6
通讯作者：
Li JG
中科院分区：
文献类型：
--
作者：
Kuang JF;Wu JY;Zhong HY;Li CQ;Chen JY;Lu WJ;Li JG
生长素是一种重要的植物激素，调节多种生理和发育过程。它通过许多生长素相关基因的转录调控参与果实脱落，包括早期生长素反应基因(即生长素/吲哚-3-乙酸(AUX/IAA)、Gretchen Hagen3(GH3)和小生长素上调(SAUR))和生长素反应因子(ARF)，这些基因在许多植物中都有很好的特征。本研究从荔枝果实中分离到5个生长素相关基因，包括1个AUX/IAA(LcAUX/IAA1)、1个GH3(LcGH3.1)、1个SAUR(LcSAUR1)和2个ARF(LcARF1和LcARF2)。LcAUX/IAA1、LcGH3.1、LcSAUR1、LcARF1和LcARF2分别含有203、613、142、792和832个氨基酸的开放阅读框(ORF)，其相对分子质量分别为22.67、69.20、11.40、88.20和93.16 kDa。在环剥+去叶处理下，由于碳水化合物运输受阻，内源IAA含量降低，加剧了荔枝果实脱落，从而研究了这些基因的表达。结果表明，LcAUX/IAA1、LcGH3.1和LcSAUR1mRNAs在离区和其他组织中的转录水平升高，而LcARF1的转录水平下降，提示LcAUX/IAA1、LcSAUR1和LcARF1可能在脱落过程中起着更重要的作用。我们的研究结果为理解碳水化合物胁迫诱导小果子脱落的过程提供了新的视角，并在分子水平上拓宽了我们对生长素信号转导途径的理解。
Auxin, a vital plant hormone, regulates a variety of physiological and developmental processes. It is involved in fruit abscission through transcriptional regulation of many auxin-related genes, including early auxin responsive genes (i.e., auxin/indole-3-acetic acid (AUX/IAA), Gretchen Hagen3 (GH3) and small auxin upregulated (SAUR)) and auxin response factors (ARF), which have been well characterized in many plants. In this study, totally five auxin-related genes, including one AUX/IAA (LcAUX/IAA1), one GH3 (LcGH3.1), one SAUR (LcSAUR1) and two ARFs (LcARF1 and LcARF2), were isolated and characterized from litchi fruit. LcAUX/IAA1, LcGH3.1, LcSAUR1, LcARF1 and LcARF2 contain open reading frames (ORFs) encoding polypeptides of 203, 613, 142, 792 and 832 amino acids, respectively, with their corresponding molecular weights of 22.67, 69.20, 11.40, 88.20 and 93.16 kDa. Expression of these genes was investigated under the treatment of girdling plus defoliation which aggravated litchi fruitlet abscission due to the blockage of carbohydrates transport and the reduction of endogenous IAA content. Results showed that transcript levels of LcAUX/IAA1, LcGH3.1 and LcSAUR1 mRNAs were increased after the treatment in abscission zone (AZ) and other tissues, in contrast to the decreasing accumulation of LcARF1 mRNA, suggesting that LcAUX/IAA1, LcSAUR1 and LcARF1 may play more important roles in abscission. Our results provide new insight into the process of fruitlet abscission induced by carbohydrate stress and broaden our understanding of the auxin signal transduction pathway in this process at the molecular level.
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影响因子：
2.8
作者：
Liu, Du-juan;Chen, Jian-ye;Lu, Wang-jin
通讯作者：
Lu, Wang-jin
影响因子：
5.2
作者：
Obeso, JR
通讯作者：
Obeso, JR
影响因子：
5.1
作者：
Liscum, E;Reed, JW
通讯作者：
Reed, JW
影响因子：
11.6
作者：
GILLASPY, G;BENDAVID, H;GRUISSEM, W
通讯作者：
GRUISSEM, W
影响因子：
11.6
作者：
Okushima, Y;Overvoorde, PJ;Theologis, A
通讯作者：
Theologis, A