An Arabidopsis ATPase gene involved in nematode-induced syncytium development and abiotic stress responses.
An Arabidopsis ATPase gene involved in nematode-induced syncytium development and abiotic stress responses.
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DOI：
10.1111/tpj.12170
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发表时间：
2013-06
期刊：
影响因子：
--
通讯作者：
Bohlmann H
中科院分区：
文献类型：
--
作者：
Ali MA;Plattner S;Radakovic Z;Wieczorek K;Elashry A;Grundler FM;Ammelburg M;Siddique S;Bohlmann H
甜菜胞囊线虫Heterodera schachtii在拟南芥根部诱导合胞体，这是其唯一的营养源。通过RT-PCR、定量RT-PCR、原位RT-PCR和启动子：：GUS检测发现，At 1g 64110在合胞体中表达上调，编码AAA+型ATP酶。合胞体中两个相关基因At 4g 28000和At 5g 52882的表达未检测到或与对照根段没有差异。使用amiRNA线和T-DNA突变体，我们表明，At 1g 64110是重要的合胞体和线虫的发展。At 1g 64110也可诱导受伤，茉莉酸，水杨酸，热和冷，以及干旱，氯化钠，脱落酸和甘露醇，表明该基因参与非生物胁迫反应。我们证实了这一点，使用两个T-DNA突变体，更敏感的脱落酸和氯化钠在种子萌发和根的生长。这些突变体还开发了显着较小的根响应脱落酸和氯化钠。计算机模拟分析表明，ATP酶At 1g 64110（以及At 4g 28000和At 5g 52882）属于AAA蛋白的“减数分裂进化枝”，包括Vps 4、katanin、spastin和MSP 1等蛋白质。
The beet cyst nematode Heterodera schachtii induces syncytia in the roots of Arabidopsis thaliana, which are its only nutrient source. One gene, At1g64110, that is strongly up-regulated in syncytia as shown by RT-PCR, quantitative RT-PCR, in situ RT-PCR and promoter::GUS lines, encodes an AAA+-type ATPase. Expression of two related genes in syncytia, At4g28000 and At5g52882, was not detected or not different from control root segments. Using amiRNA lines and T-DNA mutants, we show that At1g64110 is important for syncytium and nematode development. At1g64110 was also inducible by wounding, jasmonic acid, salicylic acid, heat and cold, as well as drought, sodium chloride, abscisic acid and mannitol, indicating involvement of this gene in abiotic stress responses. We confirmed this using two T-DNA mutants that were more sensitive to abscisic acid and sodium chloride during seed germination and root growth. These mutants also developed significantly smaller roots in response to abscisic acid and sodium chloride. An in silico analysis showed that ATPase At1g64110 (and also At4g28000 and At5g52882) belong to the ‘meiotic clade’ of AAA proteins that includes proteins such as Vps4, katanin, spastin and MSP1.
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影响因子：
5.1
作者：
Logemann E;Birkenbihl RP;Ülker B;Somssich IE
通讯作者：
Somssich IE
影响因子：
2.9
作者：
Golinowski, W;Grundler, FMW;Sobczak, M
通讯作者：
Sobczak, M
影响因子：
7.2
作者：
Niebel, A;Engler, JD;Gheysen, G
通讯作者：
Gheysen, G
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
膜变形AAA ATPase VPS4的结构和功能。
DOI：
10.1016/j.bbamcr.2011.08.017
发表时间：
2012-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Hill CP;Babst M
通讯作者：
Babst M